肠道菌群失调促进小鼠肺树突状细胞成熟并增强TLR2和TLR4表达
发布时间：2018-07-31 10:52
【摘要】：目的观察卵清蛋白(OVA)雾化吸入激发对抗生素所致肠道菌群失调小鼠肺组织中树突状细胞(DC)成熟度和Toll样受体2(TLR2)、TLR4表达的影响。方法将64只3周龄雌性BALB/c小鼠随机分为4组,菌群失调组、菌群失调加激发组、激发组、对照组,每组16只。于实验第14天用流式细胞术检测肺组织中DC成熟度(MHC-Ⅱ类分子)及其膜表面TLR2、TLR4的表达水平,采用免疫组织化学法检测肺组织中转化生长因子β1(TGF-β1)、白细胞介素-6(IL-6)蛋白表达,ELISA检测支气管肺泡灌洗液(BALF)中TGF-β1、IL-6的产生水平。结果与对照组和激发组比较,菌群失调组及菌群失调加激发组小鼠肺组织中DC细胞膜表面MHC-Ⅱ类分子及TLR2、TLR4的表达均增加,但这些分子在上述2组中的表达无差异(P0.05)。与其他3组比较,菌群失调加激发组小鼠肺组织及BALF中TGF-β1、IL-6水平增高。结论肠道菌群失调使肺部DC成熟度增高,TLR2、TLR4表达增强。
[Abstract]:Objective to investigate the effects of aerosol inhalation of ovalbumin (OVA) on the (DC) maturity of dendritic cells and the expression of Toll like receptor 2 (TLR2) TLR4 in the lung tissues of mice with intestinal dysbacteriosis induced by antibiotics. Methods Sixty-four 3-week-old female BALB/c mice were randomly divided into 4 groups, with 16 in each group. On the 14th day of the experiment, the expression of DC maturity (MHC- 鈪，
本文编号：2155360
[Abstract]:Objective to investigate the effects of aerosol inhalation of ovalbumin (OVA) on the (DC) maturity of dendritic cells and the expression of Toll like receptor 2 (TLR2) TLR4 in the lung tissues of mice with intestinal dysbacteriosis induced by antibiotics. Methods Sixty-four 3-week-old female BALB/c mice were randomly divided into 4 groups, with 16 in each group. On the 14th day of the experiment, the expression of DC maturity (MHC- 鈪，
本文编号：2155360
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