乳腺癌转移瘤生物发光动物模型复制的实验研究

发布时间：2018-11-18 08:33

【摘要】：目的复制尾静脉注射和腹腔注射接种的裸鼠乳腺癌转移瘤模型,应用生物发光技术监测肿瘤细胞在活体内的生长和转移。方法培养转染有荧光素酶基因的人乳腺癌细胞系MDA-MB-231-luc-D3-h2LN,分别通过尾静脉和腹腔接种到裸鼠体内,每周进行生物发光成像,最后解剖裸鼠体外观察转移灶。结果尾静脉接种模型肿瘤细胞在体内1周时有明显减少,之后在肺部定植并迅速增长,于接种后5周体外成像,确认肺部转移,其他部位未见肿瘤转移灶;腹腔接种模型的肿瘤细胞在腹腔形成多个转移灶,肿瘤细胞持续增殖,于接种后6周体外成像,发现壁层腹膜和脏层腹膜内多个转移灶,各脏器和腹腔以外部位未见肿瘤转移。结论建立了两种裸鼠转移瘤模型,并应用生物发光成像技术实现了深层部位肿瘤的追踪监测。
[Abstract]:Objective to establish a nude mouse model of breast cancer metastasis by tail vein injection and intraperitoneal injection, and to monitor the growth and metastasis of tumor cells in vivo by bioluminescence technique. Methods Human breast cancer cell line MDA-MB-231-luc-D3-h2LN, transfected with luciferase gene was inoculated into nude mice via tail vein and intraperitoneal cavity respectively. Bioluminescence imaging was performed every week. Finally, the metastatic foci of nude mice were observed in vitro. Results the tumor cells of caudal vein inoculation model decreased obviously at 1 week in vivo, then were colonized and rapidly increased in lung. After 5 weeks of inoculation, tumor metastasis was confirmed by imaging in vitro, but no tumor metastasis was found in other parts. The tumor cells of the peritoneal inoculation model formed multiple metastatic foci in the abdominal cavity, and the tumor cells proliferated continuously. After 6 weeks of inoculation, imaging in vitro showed that there were multiple metastatic foci in the parietal peritoneum and the visceral peritoneum, and no metastasis was found in all organs and outside the abdominal cavity. Conclusion two models of metastatic tumor in nude mice were established, and bioluminescent imaging was used to monitor the deep tumor.
【作者单位】：北京大学医药卫生分析中心分子影像学实验室;
【分类号】：R737.9;R-332

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