一株分离自美国龙虾的麦氏弧菌的鉴定及其低温状态下细胞脂肪酸的变化

发布时间：2018-12-20 06:19

【摘要】：【目的】对从美国龙虾(Homarus americanus)中分离的菌株F5-1进行种属鉴定并分析其低温状态下脂肪酸组成变化。【方法】通过VITEK 2 Compact全自动微生物鉴定仪分析菌株的生理生化特征和进行药敏试验;16S rRNA基因序列同源性分析确定该菌株的系统发育学地位;通过气相色谱和质谱联用法(GC-MS)分析菌株的全细胞脂肪酸。【结果】菌株F5-1为革兰氏阴性菌,对弧菌抑制剂O/129敏感,对青霉素有耐药性;生理生化特征与麦氏弧菌(Vibrio metschnikovii)的相似性为96%,16S rRNA序列与麦氏弧菌(GenBankNo.HQ658055)的相似性为99%;菌株的主要脂肪酸组成为C12∶0、C14∶0、C16∶0和C16∶1(n-7),不饱和脂肪酸(棕榈油酸)相对含量达34%,低温培养状态下,不饱和脂肪酸(棕榈油酸)相对含量增加至40%。【结论】将美国龙虾中分离的菌株F5-1鉴定为麦氏弧菌,该菌对多种药物敏感,菌细胞脂肪酸组成与来源于俄罗斯海参威某饮用水水库中分离的麦氏弧菌有较大差异。
[Abstract]:[objective] to identify the species of strain F5-1 isolated from American lobster (Homarus americanus) and analyze its fatty acid composition at low temperature. [methods] the strain was analyzed by VITEK 2 Compact automatic microbiological identification instrument. Physiological and biochemical characteristics and drug sensitivity test; The phylogenetic status of the strain was confirmed by homology analysis of 16s rRNA gene. The whole cell fatty acids of the strain were analyzed by gas chromatography and mass spectrometry (GC-MS). [results] strain F5-1 was Gram-negative, sensitive to Vibrio inhibitor O / 129, and resistant to penicillin. The similarity between physiological and biochemical characteristics and (Vibrio metschnikovii) of Vibrio myceli was 96s / 16s rRNA sequence and 99g / 99V / GenBankNo.HQ658055 respectively. The main fatty acid compositions of the strain were C12: 0, C14: 0, C16: 0 and C16: 1 (n-7). The relative content of unsaturated fatty acids (palmitic acid) reached 34%. [conclusion] the strain F5-1 isolated from American lobster was identified as Vibrio mycelia, which was sensitive to many drugs, and the relative content of unsaturated fatty acid (palmitic oil acid) was increased to 40. [conclusion] the strain F5-1 isolated from American lobster was identified as Vibrio mycelia. The fatty acid composition of bacteria cells was different from that of Vibrio maleicus isolated from a drinking water reservoir in the Russian sea cucumber.
【作者单位】：山东出入境检验检疫局检验检疫技术中心;辽宁出入境检验检疫局检验检疫技术中心;兰州理工大学石油化工学院;
【基金】：国家质检总局科研项目(2009IK176,2012IK305)~~
【分类号】：S941;R378

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