动态增强MRI定量监测血管阻断剂治疗兔VX2种植性肝癌的效果

发布时间：2019-01-24 19:49

【摘要】：目的评价采用动态增强MRI(DCE-MRI)定量监测血管阻断剂治疗兔VX2种植性肝癌的效果。方法成功建立28只兔VX2种植性肝癌模型,以数字法随机等分为2组,分别给予血管阻断剂M410(治疗组)和生理盐水(对照组)。给药前(基线时间点baseline)、给药后4h、1天、4天、7天、14天分别行MR平扫及DCE-MRI,并于每个时间点取肿瘤组织行病理检查(HE及CD34免疫组化染色),观察两组肿瘤体积、对比剂容积转移常量(Ktrans)、各时间点肿瘤Ktrans变化率(ΔKtrans)及病理结果的变化。结果治疗组肿瘤较对照组生长缓慢,给药后第4天、7天、14天,两组肿瘤体积差异有统计学意义(P0.05)。治疗组Ktrans值于给药4h后降低,至4天达最低,随后逐渐恢复,于14天时接近处理前水平。给药后4h、1天、4天治疗组的Ktrans值较对照组明显降低(P0.05),余各时间点Ktrans值两组差异均无统计学意义(P0.05)。两组各时间点ΔKtrans%变化趋势与Ktrans值变化趋势相同。DCE-MRI血管功能参数值改变与肿瘤组织HE及CD34染色结果相符。结论 DCE-MRI可动态定量评价血管阻断剂治疗兔VX2种植性肝癌后肿瘤组织的变化,是肿瘤血管靶向治疗后监测和评价疗效的理想的影像学手段。
[Abstract]:Objective to evaluate the effect of dynamic enhanced MRI (DCE-MRI) quantitative monitoring vascular blocker in the treatment of VX2 implanted hepatocellular carcinoma in rabbits. Methods 28 VX2 implanted liver cancer models were successfully established in 28 rabbits. The models were randomly divided into two groups by digital method. M410 (treatment group) and normal saline (control group) were given vascular blocker M410 (treatment group) and normal saline (control group) respectively. MR plain scan and DCE-MRI, were performed on 1 day, 4 days, 7 days and 14 days after administration of baseline), at baseline time point, and pathological examination (HE and CD34 immunohistochemical staining) were performed at each time point to observe the tumor volume of the two groups. The change rate of Ktrans (螖 Ktrans) and pathological results of tumor at different time points of (Ktrans), were compared. Results the tumor growth in the treatment group was slower than that in the control group. There was a significant difference in tumor volume between the two groups on the 4th, 7th and 14th day after administration (P0.05). In the treatment group, the Ktrans value decreased 4 hours after administration, reached the lowest level at 4 days, then recovered gradually, and was close to the level before treatment at 14 days. The Ktrans value of the treatment group was significantly lower than that of the control group on the 1st day and 4th day after administration (P0.05). There was no significant difference in the Ktrans value between the two groups at the remaining time points (P0.05). The change trend of 螖 Ktrans% was the same as that of Ktrans at each time point in both groups. The changes of DCE-MRI vascular function parameters were consistent with the results of HE and CD34 staining in tumor tissues. Conclusion DCE-MRI can dynamically and quantitatively evaluate the changes of tumor tissue after VX2 implanted hepatocarcinoma in rabbits, and it is an ideal imaging method for monitoring and evaluating the therapeutic effect of vascular targeting therapy.
【作者单位】：暨南大学附属第一医院医学影像中心;中国科学院广州化学研究所;广州医科大学附属广州市第一人民医院放射科;广州医科大学附属广州市第一人民医院病理科;
【基金】：国家自然科学基金青年科学基金项目(81201087) 广东省自然科学基金(10151006001000010) 广州市科技攻关项目(2010Y1-C361)
【分类号】：R-332

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