鸡法氏囊病毒多表位抗原的表达及免疫特性分析
发布时间：2019-02-16 18:24
【摘要】：为进行传染性法氏囊病病毒(IBDV)多表位抗原的原核表达和免疫特性分析,应用重叠延伸PCR技术扩增合成由病毒VP2和VP3蛋白多个表位序列构成的重组抗原基因,并将抗原基因与原核表达载体pBVIL1重组,表达多表位融合抗原,通过与标准抗血清反应和动物免疫分别检测表达抗原的免疫反应性和免疫原性。结果显示,经42℃诱导后,含重组载体的大肠杆菌表达了31 kD的抗原蛋白,Western blot鉴定出现阳性条带,免疫小鼠后,抗血清的滴度为1∶6 400。说明原核表达载体构建成功,大量表达的多表位融合抗原具有IBDV抗原反应性。
[Abstract]:In order to analyze the prokaryotic expression and immunological characteristics of (IBDV) polyepitope antigen of infectious bursal disease virus (IBDV), a recombinant antigen gene composed of multiple epitopes of VP2 and VP3 proteins was synthesized by overlapping extended PCR technique. The antigen gene was recombined with prokaryotic expression vector pBVIL1 to express multi-epitope fusion antigen. The immunoreactivity and immunogenicity of the expressed antigen were detected by reaction with standard antiserum and animal immunity. The results showed that after induction at 42 鈩，
本文编号：2424717
[Abstract]:In order to analyze the prokaryotic expression and immunological characteristics of (IBDV) polyepitope antigen of infectious bursal disease virus (IBDV), a recombinant antigen gene composed of multiple epitopes of VP2 and VP3 proteins was synthesized by overlapping extended PCR technique. The antigen gene was recombined with prokaryotic expression vector pBVIL1 to express multi-epitope fusion antigen. The immunoreactivity and immunogenicity of the expressed antigen were detected by reaction with standard antiserum and animal immunity. The results showed that after induction at 42 鈩，
本文编号：2424717
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