游离脂肪酸对肝细胞ACSL1表达及相关脂代谢的影响

发布时间：2019-08-12 10:02

【摘要】：目的研究游离脂肪酸(FFA)的诱导对L02肝细胞长链脂酰CoA合成酶1(ACSL1)的表达及相关代谢的影响。方法用含不同浓度(0.2、0.4、0.8 mmol/L)FFA的培养液诱导L02细胞48 h,Western blot检测ACSL1蛋白水平,荧光定量PCR检测ACSL1 mRNA水平,比色法测定甘油三酯(TG)含量、ATP水平和培养上清FFA浓度变化,生化法测定酮体含量和培养上清葡萄糖浓度变化。结果 FFA的诱导可显著提高ACSL1蛋白表达水平(P0.01),但对ACSL1 mRNA水平无明显影响(P0.05),细胞内TG含量显著升高(P0.01或P0.05),酮体含量显著升高(P0.05),培养上清葡萄糖消耗显著增加(P0.01),胞内ATP水平无明显变化(P0.05),与0.2 mmol/L、0.4 mmol/L FFA组相比,0.8 mmol/L FFA组培养上清FFA消耗显著增加(P0.01或P0.05)。结论 FFA通过上调ACSL1蛋白表达水平致肝细胞TG蓄积。
[Abstract]:Objective to study the effect of free fatty acid (FFA) induction on the expression and related metabolism of long chain acyl CoA Synthase 1 (ACSL1) in L02 hepatocytes. Methods L02 cells were induced with different concentrations of FFA (0.2, 0.4, 0.8 mmol/L) for 48 hours. Western blot detected the level of ACSL1 protein, fluorescence quantitative PCR detected the level of ACSL1 mRNA, colorimetric assay was used to determine the content of glycerol (TG), the level of ATP and the concentration of FFA in culture. The content of ketosome and the concentration of glucose in culture were measured by biochemical method. Results the expression of ACSL1 protein was significantly increased by FFA induction (P01), but there was no significant effect on ACSL1 mRNA level (P05). The content of TG in cells was significantly increased (P01 or P05), the content of ketosome was significantly increased (P05), the glucose consumption of culture supernatant was significantly increased (P01), but the level of intracellular ATP was not significantly changed (P05). In 0.8 mmol/L FFA group, FFA consumption in culture medium was significantly higher than that in control group (P 0.01 or P 0.05). Conclusion FFA can induce TG accumulation in hepatocytes by up-regulating the expression of ACSL1 protein.
【作者单位】：福建中医药大学中西医结合学院;
【基金】：福建省自然科学基金(No.2011J01207)
【分类号】：R341

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