一种新发现的、睾丸高表达的非编码RNA,RHNO1在紫外线引起的DNA双链断裂修复过程中的作用

发布时间：2021-05-31 23:30

　　目的:发现一个转录来自RHNO1基因的长链非编码RNA（IncRNA）并分析其在脱氧核糖核酸双链断裂（DSB）修复中的功能。方法:以过表达质粒为基础,运用免疫印迹、Real-time PCR和LC/MSMS共同验证RHNO1基因的转录、翻译情况。通过Real-time PCR分析了RHNO1在小鼠各组织中的转录水平的表达情况。通过原位杂交法分析RHNO1在生精周期中的表达情况。以UV照射引起的生殖细胞系DSB为模型,进一步验证RHNO1在DSB修复中的作用。结果:全长RHNO1基因可以表达IncRNA。RHNO1基因在小鼠睾丸组织中表达最高并主要定位在精母细胞和圆形精子中。RHNO1在生殖细胞系中具有DSB修复作用。结论:发现了新的长链非编码RNA,RHNO1,其在小鼠睾丸组织中高表达,并在生殖细胞系中具有DNA损伤修复作用。 

【文章来源】：中华男科学杂志. 2020,26(05)北大核心CSCD

【文章页数】：7 页

【部分图文】：

RHNO1是一个新的长链非编码RNA (n=3)

表1 LC/MSMS质谱检出蛋白对应基因(本表列出Score前50的SDS-PAGE凝胶检出蛋白所对应基因)Table 1. Genes corresponding to the 50 proteins with the highest scores detected by SDS-PAGE gel electrophoresis Genes in the full-length RHNO1 group Gene ID Score Genes in the control group Gene ID Score 14-3-3 protein epsilon P62259 912 Eukaryotic translation initiation factor 5A-1 P63242 881 Guanine nucleotide-binding protein subunit beta-2-like 1 P68040 689 14-3-3 protein epsilon P62259 860 Eukaryotic translation initiation factor 5A-1 P63242 661 Guanine nucleotide-binding protein subunit beta-2-like 1 P68040 690 Ubiquitin-40S ribosomal protein S27a P62983 595 Ubiquitin-40S ribosomal protein S27a P62983 609 Nucleoside diphosphate kinase E9PZF0 475 40S ribosomal protein S3 P62908 463 60S ribosomal protein L37a P61514 472 40S ribosomal protein S2 P25444 444 60S ribosomal protein L7a P12970 461 Histone H1.2 P15864 375 Small nuclear ribonucleoprotein-associated protein B P27048 456 Nucleoside diphosphate kinase E9PZF0 364 60S ribosomal protein L9 P51410 370 Voltage-dependent anion-selective channel protein 2 (Fragment) G3UX26 350 Spectrin alpha chain, erythrocytic 1 P08032 370 Peptidyl-prolyl cis-trans isomerase A P17742 336 Tropomyosin alpha-3 chain D3Z6I8 363 Proteasome subunit alpha type-4 Q9R1P0 330 Pyrroline-5-carboxylate reductase 2 Q922Q4 357 Histone H2B type 1-P Q8CGP2 307 40S ribosomal protein S8 P62242 344 Histone H4 P62806 306 Triosephosphate isomerase P17751 344 Phosphoglycerate mutase 1 Q9DBJ1 305 40S ribosomal protein S3 P62908 338 Cofilin-1 P18760 301 Histone H1.3 P43277 327 60S ribosomal protein L7a P12970 300 40S ribosomal protein S14 (Fragment) P62908 326 Triosephosphate isomerase P17751 297 60S ribosomal protein L10a Q5XJF6 325 Tropomyosin alpha-3 chain D3Z6I8 295 40S ribosomal protein S2 P25444 314 Pyrroline-5-carboxylate reductase 2 Q922Q4 295 High mobility group protein B1 P63158 309 ADP/ATP translocase 2 P51881 291 Histone H2B type 1-B Q64475 303 40S ribosomal protein S4, X isoform P62702 284 Cofilin-1 P18760 303 Serine/arginine-rich splicing factor 2 Q62093 283 ADP/ATP translocase 2 P51881 289 Capping protein (Actin filament) muscle Z-line, beta, isoform A2AMW0 273 Proteasome subunit alpha type-4 Q9R1P0 287 Small nuclear ribonucleoprotein-associated protein B P27048 267 Band 3 anion transport protein P04919 279 60S ribosomal protein L8 P62918 262 Proteasome activator complex subunit 3 P61290 273 Transcription factor BTF3 Q64152 252 Peptidyl-prolyl cis-trans isomerase A P17742 272 Proteasome activator complex subunit 3 P61290 239 Phosphoglycerate mutase 1 Q9DBJ1 245 60S ribosomal protein L9 P62918 237 60S ribosomal protein L18a P62702 244 Proteasome subunit alpha type-7 Q9Z2U0 237 Voltage-dependent anion-selective channel protein 1 Q60932 217 Malignant T-cell-amplified sequence 1 Q9DB27 235 40S ribosomal protein S4, X isoform P62702 215 60S ribosomal protein L18a P62717 226 Heterogeneous nuclear ribonucleoprotein A1 Q5EBP8 213 Protein Gm9493 F6SVV1 223 60S ribosomal protein L8 P62918 208 40S ribosomal protein S14 P62264 220 Proteasome subunit beta type-3 Q9R1P1 200 Chloride intracellular channel protein 4 Q9QYB1 220 Histone H4 P62806 198 Cbx3 protein Q9DCC5 219 Malignant T-cell-amplified sequence 1 Q9DB27 197 Rho GDP-dissociation inhibitor 1 Q99PT1 218 Myosin light polypeptide 6 Q60605 197 Peptidyl-prolyl cis-trans isomerase B P24369 205 Exosome complex component RR P41Q921I9 195 Proteasome subunit alpha type-5 Q9Z2U1 203 Small nuclear ribonucleoprotein F P62307 195 Histone H2A type 3 Q8BFU2 202 40S ribosomal protein S12 Q6ZWZ6 194 Small nuclear ribonucleoprotein F P62307 202 Serine/arginine-rich splicing factor 2 Q62093 193 Cellular nucleic acid-binding protein P53996 201 Protein Gm9493 F6SVV1 192 60S ribosomal protein L17 Q9CPR4 199 60S ribosomal protein L26 B1ARA5 192 60S ribosomal protein L10a Q5XJF6 197 Adenylate kinase 2, mitochondrial Q9WTP6 191 N-alpha-acetyltransferase 50 Q6PGB6 195 Cbx1 protein Q7TPM0 191 Small nuclear ribonucleoprotein Sm D2 P62317 191 14-3-3 protein beta/alpha Q9CQV8 190 40S ribosomal protein S15 P62843 190 GTP-binding nuclear protein Ran P62827 189 60S ribosomal protein L26 (Fragment) B1ARA3 187 Heat shock 70 kDa protein 1B P17879 189 40S ribosomal protein S24 P62849 187 40S ribosomal protein S10 P63325 187 Peroxiredoxin-4 O08807 182 RNA-binding protein 8A A0A023T672 184 Proteasome subunit alpha type-1 Q9R1P4 182图3 RHNO1在小鼠生殖细胞系GC1-spg中的DSB修复作用

RHNO1在小鼠生殖细胞系GC1-spg中的DSB修复作用

【参考文献】：
期刊论文
[1]RAD51调控REV1参与DNA双链断裂修复[J]. 黄敏,杨业然,孙晓艳,张婷,郭彩霞.  遗传. 2018(11)
[2]减数分裂重组关键蛋白MLH1结合蛋白的酵母双杂交筛选和鉴定[J]. 殷毅,平萍.  中国男科学杂志. 2016(01)



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