深低温停循环对大鼠海马蛋白激酶CK2α表达的影响

发布时间：2017-12-31 08:36

本文关键词：深低温停循环对大鼠海马蛋白激酶CK2α表达的影响　出处：《昆明医科大学》2014年硕士论文　论文类型：学位论文

【摘要】：[目的]深低温停循环对大鼠海马蛋白激酶CK2α表达的影响和深低温脑保护的可能机制。 [方法]取SD大鼠30只,随机分为3组：深低温停循环(DHCA)组、常温停循环(NTCA)组和正常对照(NC)组(N=10)。采用体外插管法建立大鼠闭胸式体外循环模型,DHCA组体外循环建立后在深低温(27℃)条件下停循环10分钟；NTCA组建立体外循环后在正常体温(36.8℃～37℃)条件下停循环10分钟；NC组建立体外循环后不进行降温和停循环过程,保持正常体温(36.8℃-37℃)体外循环20分钟。其后迅速断头取大鼠大脑双侧海马组织,标本制作成冰冻切片和石蜡切片,应用免疫荧光染色和免疫组化法从蛋白质水平研究蛋白激酶CK2α在缺血早期大鼠大脑海马组织的表达情况。结果采用SPSS13.0软件进行统计学分析。 [结果]蛋白激酶CK2a在深低温停循环组和常温停循环组较正常对照组表达均有明显升高,差异有统计学意义(P0.05)；蛋白激酶CK2α在常温停循环组较正常对照组表达明显增高,差异有统计学意义(P0.05)；蛋白激酶CK2α在深低温全脑缺血组较常温停循环组表达明显降低,差异有统计学意义(P0.05)。 [结论]缺氧可诱导大鼠大脑海马蛋白激酶CK2α的表达,而深低温可以在相同的缺血缺氧条件下蛋白激酶CK2α的表达下降,从而发挥缺血缺氧下的脑保护作用。深低温降低蛋白激酶CK2α的表达可能是深低温脑保护机制之一。
[Abstract]:Objective To investigate the effects of deep and low temperature circulatory arrest on the expression of protein kinase CK2伪 in rat hippocampus and the possible mechanism of deep hypothermia brain protection . Methods Thirty - three SD rats were randomly divided into three groups : deep low temperature circulatory arrest ( DHCA ) group , normal temperature arrest cycle ( NTCA ) group and normal control ( NC ) group ( NC ) group ( N = 10 ) . Compared with the normal control group , there was a significant difference in the expression of protein kinase CK2a ( P0.05 ) , and the expression of protein kinase CK2伪 at normal temperature was significantly lower than that in normal control group ( P0.05 ) . Conclusion Hypoxia can induce the expression of protein kinase CK2伪 in the rat brain , while the deep and low temperature can decrease the expression of protein kinase CK2伪 under the same conditions of ischemia and hypoxia , thereby playing the role of brain protection under ischemia and hypoxia . The reduction of protein kinase CK2伪 in deep hypothermia may be one of the mechanisms of deep hypothermia brain protection .

【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R651.15

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