骨腱结合部急性损伤后愈合过程的组织形态学观察

发布时间：2018-01-28 08:01

本文关键词： 针刺损伤骨腱结合部损伤愈合　出处：《北京体育大学》2013年硕士论文　论文类型：学位论文

【摘要】：骨腱结合部损伤是运动中常见的损伤,严重地影响运动员的运动能力和运动寿命。近年来对骨腱结合部慢性损伤地研究比较多,但非断裂性急性损伤得不到合理地愈合往往能够引起慢性损伤的发生。本实验采用梅花针针刺兔髌骨骨腱结合部制造急性非断裂损伤,观察损伤后组织的愈合情况,试图找到愈合所需地时间,为伤后合理地休息时间提供依据。本实验研究对象为30只成年雌性新西兰大白兔(18周龄,体重2.85士0.2千克)。随机筛选分为5组：对照组(CON,n=6)；实验组(N,n=24)：针刺损伤愈合1周(ND1W,n=6),针刺损伤愈合2周(ND2W,n=6),针刺损伤愈合4周(ND4W,n=6),针刺损伤愈合8周(ND8W,n=6)。对照组动物不做处理；实验组动物在其右侧后肢髌骨下缘髌腱附丽处进行针刺。实验组分别1,2,4,8周取材,对照组在8周取材,组织脱钙,脱水,石蜡包埋,切片,HE染色,Safranin0染色,VEGF, bFGF免疫组化染色。进行骨腱结合部愈合的不同时间段的组织学观察,对显微镜下所采集的图像进行测量和统计学分析。结果：ND1W与CON组相比,可观察到骨腱结合部细胞排列紊乱,潮线不清晰,有血管入侵,瘢痕组织形成,软骨细胞明显增加,组织肌腱纤维和腱细胞排列紊乱。ND2W组仍可见骨腱结合部细胞排列不均,血管增生,瘢痕组织,潮线不清晰,组织胶原纤维排列仍然紊乱,腱细胞排列不均。ND4W组可见骨腱结合部细胞排列较整齐,可观察到较清晰的潮线,但潮线的弧度不是与髌骨弧度大体一致,而是向肌腱内推进,组织胶原纤维排列稍显整齐,但仍可见腱细胞团聚。ND8W组可见骨腱结合部细胞排列较整齐,纤维软骨区已经可以观察到相互交叉的Sharpey纤维,潮线明显,其弧度与髌骨轮廓大体一致,但仍可见轻微涨潮显现,组织胶原纤维排列较为整齐。ND1W,ND2W组细胞密度明显增加,而ND4W,ND8W与CON组无显著差别。纤维软骨带厚度直到损伤8周是仍明显高于CON组,VEGF和bFGF的表达在ND1W,ND2W组都明显增加,而在ND2W,ND2W组恢复正常水平。结论：①在损伤八周时,纤维软骨带的重建并没有结束,提示骨腱结合部纤维软骨带愈合的困难性。②骨腱结合部损伤的愈合过程在第4周后有所停滞。
[Abstract]:Bone tendon joint injury is a common injury in exercise, which seriously affects athletes' motor ability and exercise life. In recent years, there are more researches on the chronic injury of bone tendon joint. However, chronic injury can often occur if the non-rupture acute injury is not properly healed. In this experiment, the rabbit patellar tendon joint was treated with plum blossom needle to make the acute non-rupture injury. To observe the healing of tissue after injury, to find out the time needed for healing, and to provide the basis for reasonable rest time after injury. 30 adult female New Zealand white rabbits were studied in this study at the age of 18 weeks. The weight was 2.85 卤0.2 kg / kg. The control group was randomly divided into five groups: control group (control group), Conn (n = 6), and control group (n = 6). In the experimental group, the acupuncture wound healed 1 week, the acupuncture wound healed 2 weeks, and the acupuncture wound healed 4 weeks later. The acupuncture wound healed for 8 weeks and the control group was not treated. The experimental group was treated with acupuncture on the patellar tendon attached to the lower edge of the patella in the right hind limb. The experimental group was taken from the patellar tendon at 1: 2 and 4 weeks, while the control group was taken at 8 weeks, the tissue was decalcified, dehydrated, paraffin embedded and sectioned. The histopathological changes of bone tendon union were observed at different time stages by HE staining, Safranin 0 staining and bFGF immunohistochemical staining. Results compared with the CON group, the bone tendon junction cells were disordered, the tidal lines were not clear and the blood vessel invasion was observed in the W / ND 1W group. Scar tissue formation, chondrocyte increase significantly, tissue tendon fiber and tendon cell arrangement disorder. ND2W group can still see bone tendon junction cells arranged unevenly, vascular proliferation, scar tissue, tidal line is not clear. The arrangement of collagen fibers in tissue was still disordered, and the arrangement of tendon cells was not uniform in group .ND4W, and a clear tidal line could be observed, but the curve of tidal line was not roughly the same as that of patella. Instead of advancing into the tendon, the collagenous fibers in the tissue were arranged neatly, but the tendon cells could still be seen in the bone tendon junction cells in the group .ND8W. The intersecting Sharpey fibers can be observed in the fibrous cartilage region. The tidal lines are obvious and the radians are approximately the same as the patellar contours, but the slight tidal rise can still be seen. The tissue collagen fibers arranged neatly. ND1WN 2W group significantly increased cell density, while ND4W. There was no significant difference between ND8W group and CON group. The thickness of fibrochondral band was significantly higher than that of CON group until the 8th week of injury. The expression of VEGF and bFGF in ND1W / Nd2W group was significantly higher than that in Nd-1W / Nd2W group. But in ND2WN 2W group, the normal level was restored. Conclusion the reconstruction of fibrochondrocytes is not over at 8 weeks after the injury. The results suggest that the healing process of fibrous chondrocytes in tendon junctions is difficult. 2. The healing process of bone tendon junctional injuries is at a standstill after 4 weeks.
【学位授予单位】：北京体育大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R686.1

【共引文献】