跑台运动对脑外伤大鼠基质金属蛋白酶-2表达的影响

发布时间：2018-01-30 02:48

本文关键词： 脑外伤跑台训练血脑屏障基质金属蛋白酶大鼠　出处：《中国康复理论与实践》2017年05期 　论文类型：期刊论文

【摘要】：目的观察跑台训练对脑外伤大鼠基质金属蛋白酶-2(MMP-2)表达的影响。方法 55只成年Sprague-Dawley大鼠随机分为假手术组(n=15)、对照组(n=20)和运动组(n=20),后两组复制中重度脑外伤模型,运动组进行电动跑台训练1周。术后6、12、18、24 d,采用足误试验评定运动协调功能;术后48 h,采用伊文思蓝(EB)染色检测大鼠血脑屏障通透性;术后7 d,Western blotting检测MMP-2的表达。结果运动组在术后6 d评分明显降低,但随后增高,其中12 d、18 d及24 d评分比对照组高(F4.793,P0.05);运动组EB渗出量显著减少(t=-8.091,P0.001);运动组MMP-2蛋白表达显著下降(t=-13.12,P0.001)。结论大鼠脑外伤后早期进行跑台训练,可改善运动功能,可能与抑制MMP-2,从而减轻血脑屏障破坏有关。
[Abstract]:Objective to observe the effect of treadmill training on the expression of matrix metalloproteinase-2 (MMP-2) in brain trauma rats. Methods 55 adult Sprague-Dawley rats were randomly divided into sham operation group (n = 55). 15). The model of moderate and severe brain injury was made in the latter two groups. The exercise group was trained with electric treadmill for 1 week. Foot error test was used to evaluate the function of motor coordination. At 48 hours after operation, the permeability of blood-brain barrier in rats was detected by EBB staining. The expression of MMP-2 was detected by Western blotting on the 7th day after operation. Results the score of exercise group decreased significantly on the 6th day after operation, but then increased, including 12 days. The scores of 18 days and 24 days were higher than those of the control group. In exercise group, the exudation of EB decreased significantly (P 0.001). The expression of MMP-2 protein decreased significantly in the exercise group. Conclusion the treadmill training in the early stage after brain injury can improve the motor function and may inhibit MMP-2. So as to alleviate the blood-brain barrier damage related.
【作者单位】：上海市第一康复医院康复科;复旦大学附属华山医院康复医学科;
【基金】：杨浦区科学技术委员会、卫生和计划生育委员会课题(No.YP15M13)
【分类号】：R651.15
【正文快照】： [本文著录格式]沈夏锋,田闪,吴毅,等.跑台运动对脑外伤大鼠基质金属蛋白酶-2表达的影响[J].中国康复理论与实践,2017,23(5):525-528.CITED AS:Shen XF,Tian S,Wu Y,et al.Effect of treadmill exercise on expression of matrix metalloproteinase-2 in rats with traumat-ic

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