脉冲磁场对颅脑损伤脑组织中内源性神经干细胞因子的影响

发布时间：2018-01-31 00:57

本文关键词： 干细胞神经干细胞组织工程分化内源性神经干细胞因子脉冲磁场颅脑损伤大鼠脑功能　出处：《中国组织工程研究》2017年21期 　论文类型：期刊论文

【摘要】：背景:有研究指出,脉冲电磁在促进及诱导脑梗死、脊髓损伤周围神经再生,改善神经疾病患者记忆功能等方面有良好效果。目的:探讨脉冲磁场对颅脑损伤大鼠脑功能及脑组织中内源性神经干细胞因子的影响。方法:将320只雄性成年SD大鼠随机分为模型组、脉冲磁场0.1 mT组、脉冲磁场0.3 mT组及脉冲磁场0.5 mT组,均采用侧向液压打击法建立颅脑损伤模型,脉冲磁场0.1 mT组、脉冲磁场0.3 mT组及脉冲磁场0.5 mT组建模后给予相应强度的脉冲磁场照射,照射后1,3,7,14 d,进行平衡木行走实验、水迷宫实验,以测定大鼠运动功能;相应照射时间点前1 d,腹腔注射BrdU,应用免疫组织化学法测定脑组织BrdU及皮质巢蛋白阳性细胞数。结果与结论:(1)照射后1,3,7 d,脉冲磁场0.1,0.3,0.5 mT组水迷宫实验时间、平衡木行走实验均短于模型组(P0.05),脉冲磁场0.5 mT组水迷宫实验时间、平衡木行走实验均短于脉冲磁场0.1,0.3 mT组(P0.05),脉冲磁场0.3 mT组水迷宫实验时间、平衡木行走实验均短于脉冲磁场0.1 mT组(P0.05);(2)照射后3,7,14 d,脉冲磁场0.1,0.3,0.5 mT组Brd U及皮质巢蛋白阳性细胞数多于模型组(P0.05),脉冲磁场0.5 mT组Brd U及皮质巢蛋白阳性细胞数多于脉冲磁场0.1,0.3 mT组(P0.05),脉冲磁场0.3 mT组Brd U及皮质巢蛋白阳性细胞数多于脉冲磁场0.1 mT组(P0.05);(3)结果表明,脉冲磁场能明显保护颅脑损伤大鼠脑功能,且电磁场强度越强效果越明显,其可能机制与脉冲磁场激活颅脑损伤大鼠脑组织中神经干细胞及促进其增殖有关。
[Abstract]:Background: it has been pointed out that pulse electromagnetism promotes and induces the regeneration of peripheral nerve after cerebral infarction and spinal cord injury. Objective: to investigate the effect of pulsed magnetic field on brain function and endogenous neural stem cell factor in brain tissue of rats with craniocerebral injury. 320 adult male SD rats were randomly divided into model group. The craniocerebral injury model was established by lateral hydraulic beating method in the pulse magnetic field 0.1 Mt group, the pulse magnetic field 0.3 Mt group and the pulse magnetic field 0.5 Mt group, and the pulse magnetic field 0.1 Mt group. The pulse magnetic field 0.3 Mt group and the pulse magnetic field 0.5 Mt group were treated with the corresponding intensity of pulsed magnetic field irradiation. The balance beam walking experiment and the water maze experiment were carried out on the first day after irradiation. To determine the motor function of rats; BrdU was injected intraperitoneally 1 day before the corresponding irradiation time point. The number of BrdU and cortex nestin positive cells in brain tissue was determined by immunohistochemical method. After 7 days, the experimental time of water maze and the walking experiment of balance beam in 0.1mt 0.3mT group were shorter than those in model group (P0.05). The experimental time of water maze and walking test of balance beam in 0.5 Mt group were shorter than that in 0.3 Mt group (P 0.05). The experimental time of water maze and walking test of balance beam in 0.3 Mt group were shorter than that in 0.1 Mt group. (2) the number of Brd U and nestin positive cells in 0. 1 ~ 0. 3mT group was more than that in the model group (P 0. 05). The number of Brd U and nestin positive cells in pulsed magnetic field group was more than that in 0.1mT group (P 0.05). The number of Brd U and nestin positive cells in the pulse magnetic field group was more than that in the pulse magnetic field group. The results show that pulsed magnetic field can obviously protect brain function of rats with brain injury, and the stronger the electric magnetic field is, the more obvious the effect is. The mechanism may be related to the activation and proliferation of neural stem cells in brain tissue after brain injury by pulsed magnetic field.
【作者单位】：郑州大学附属医院南阳市中心医院创伤外科;郑州大学附属医院南阳市中心医院神经外科;郑州大学附属医院南阳市中心医院临床药学科;郑州大学第一附属医院;
【分类号】：R651.15
【正文快照】： 文章快速阅读:脉冲磁场促进颅脑损伤大鼠脑组织中内源性神经干细胞的增殖结果:分组干预:检测指标:(1)脉冲磁场能明显保护(1)颅脑损伤模型;(1)照射后1,3,7,14 d,进颅脑损伤大鼠脑功能;(2)颅脑损伤模型+脉行平衡木行走实验、水迷宫实(2)且电磁场强度越强效冲磁场0.1 mT干预;验,

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