GDF-15基因多态性与急性心肌梗死侧枝循环形成的相关性分析

发布时间：2018-02-02 15:54

本文关键词： 急性心肌梗死(AMI) 生长分化因子-15(GDF-15) 基因多态性聚合酶链反应-限制性片段长度多态性(PCR-RFLP) 侧枝循环　出处：《山西医科大学》2013年硕士论文　论文类型：学位论文

【摘要】：目的：探讨生长分化因子-15(GDF-15)基因-3148C／G位点多态性与太原地区汉族人群急性心肌梗死(AMI)侧枝循环形成的关系。方法：收集92例急性心肌梗死组(血管急性闭塞3-12小时内)患者作为病例组(有侧枝组68例,无侧枝组24例)和56例冠造正常者作为对照组,两组样本均取自太原地区汉族人群。提取外周血DNA,运用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)、DNA测序及序列比对的方法分析GDF-15基因-3148C／G位点多态性与急性心肌梗死侧枝循环形成的相关性。结果：太原地区汉族人群中GDF-15基因-3148C／G位点经检测存在两种基因型：CC和GC基因型,而未检测到GG基因型。急性心肌梗死组和正常对照组人群均符合Hardy-Weinberg遗传平衡定律,具有群体代表性。在急性心肌梗死组中,CC、GC两种基因型频率分别为80.43%和19.57%,在正常对照组中分别为60.71%和39.29%；两组受试者-3148C／G位点的CC、GC基因型频率分布有统计学意义(X2=6.864,P=0.009)；风险基因型为GC型,与基因型CC型相比,携带GC基因型可使发生急性心肌梗死的风险升高2.6倍(OR=2.660,95%可信区间为1.265-5.595)。在急性心肌梗死患者血管急性闭塞3-12小时内有侧枝形成组,CC、GC基因型频率分别为85.29%和14.71%,在无侧枝形成组中,CC、GC基因型频率分别分别为66.67%和33.33%；两组受试者-3148C／G位点的CC、GC基因型频率分布有统计学意义(X2=3.911,P=0.048)；风险基因型为GC基因型,与基因型CC型相比,携带GC基因型的急性心肌梗死患者,其血管急性闭塞3-12小时内侧枝循环形成的可能性较基因型CC型增高2.9倍(0R=2.900,95%可信区间为0.983-8.556)。结论：GDF-15基因-3148C／G位点中,GC基因型携带者发生急性心肌梗死的易感性大于CC基因型携带者,同时,在急性心肌梗死患者血管闭塞3-12小时内,携带GC基因型者更易于侧枝循环的形成,检测GDF-15的基因型可作为急性心肌梗死早期诊断和防治的重要标记物。
[Abstract]:Objective: to investigate the relationship between the polymorphism of growth differentiation factor-15 (GDF-15) gene -3148C / G locus and collateral circulation formation in acute myocardial infarction (AMI) of Han nationality in Taiyuan. Methods: 92 patients with acute myocardial infarction (within 3-12 hours of acute vascular occlusion) were selected as the case group (68 cases with collateral branch group, 24 cases with no collateral branch group) and 56 cases with normal coronary artery as control group. The samples of both groups were extracted from the Han population in Taiyuan area. The peripheral blood DNA was extracted and PCR-RFLP was used to detect the DNA in the peripheral blood by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The relationship between the polymorphism of GDF-15 gene -3148C / G locus and collateral circulation in acute myocardial infarction was analyzed by DNA sequencing and sequence alignment. Results: there were two genotypes of GDF-15 gene in Taiyuan Han population: -3148C / G locus. There were two genotypes: 1. CC and GC. But no GG genotypes were detected. Both acute myocardial infarction (AMI) group and normal control group were in accordance with the law of genetic balance of Hardy-Weinberg. The frequencies of the two genotypes were 80.43% and 19.57, respectively, and 60.71% and 39.29 in the normal control group, respectively. The frequency distribution of CCG genotype at -3148C / G locus in the two groups was statistically significant (P < 0.05). Compared with CC genotype, carrying GC genotype increased the risk of acute myocardial infarction by 2.6 times. The confidence interval of 95% was 1.265-5.595 渭 m. In acute vascular occlusion patients with acute myocardial infarction within 3-12 hours, there were lateral branch forming group (CC). The frequency of GC genotype was 85.29% and 14.71, respectively. In the group without lateral branch formation, the frequency of GC genotype was 66.67% and 33.33, respectively. The frequency distribution of CCG genotype at -3148C / G locus in the two groups was statistically significant (X _ 2N _ (3.911) P ~ (0.048)); The risk genotype was GC genotype, and compared with CC genotype, acute myocardial infarction patients with GC genotype. The probability of collateral circulation formation was 2.9 times higher than that of CC genotype within 3-12 hours after acute occlusion. The 95% confidence interval of 0.983-8.556 was 0.983-8.556. Conclusion the susceptibility of GC genotype carriers to acute myocardial infarction is higher than that of CC genotype carriers. Patients with GC genotype were more likely to form collateral circulation within 3-12 hours of occlusion in patients with acute myocardial infarction. Detection of GDF-15 genotypes may be an important marker for early diagnosis and prevention of acute myocardial infarction.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R542.22

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