脑源性神经营养因子预处理神经干细胞移植对急性缺血性脑卒中小鼠的效果

发布时间：2018-02-08 14:58

本文关键词： 缺血性脑卒中神经干细胞脑源性神经营养因子体外培养移植　出处：《中国康复理论与实践》2017年11期 　论文类型：期刊论文

【摘要】：目的比较脑源性神经营养因子(BDNF)预处理对神经干细胞(NSCs)移植修复急性缺血性脑卒中的作用。方法出生1 d C57BL/6小鼠,分离NSCs体外培养及鉴定。10周龄健康C57BL/6小鼠150只随机分为5组,A组(n=20)为假手术组,B(n=20)、C(n=20)、D(n=45)、E(n=45)组采用光化学诱导法建立急性脑缺血模型。造模24 h后,D组行单纯NSCs移植,E组行BDNF预处理的NSCs移植,C组移植等量溶剂。移植前1 d,移植后3 d、7 d、14 d、21 d、28 d,行加速转棒测试及前肢抓力测试,移植后28 d,D、E组取5只小鼠行微管相关蛋白2(MAP-2)、神经胶质纤维酸性蛋白(GFAP)免疫荧光染色。结果移植后3 d、7 d、14d、21 d、28 d,加速转棒时间由长到短依次为E组、D组、B组(P0.05);移植后14 d、21 d、28 d,前肢抓力由大到小依次为E组、D组、B组(P0.05)。移植后28 d,D组、E组均发现Edu/GFAP双阳性细胞及Edu/MAP-2双阳性细胞。结论 NSCs移植可促进缺血性脑卒中后行为功能恢复,经BDNF预处理后有更好效果。
[Abstract]:Objective to compare the effects of brain-derived neurotrophic factor (BDNF) preconditioning on neural stem cell transplantation (NSCs) in the repair of acute ischemic stroke. Methods one day C57BL / 6 mice were born. A total of 150 healthy 10-week-old C57BL / 6 mice were isolated and identified in vitro. They were randomly divided into 5 groups (group A) as sham-operation group (group B) as sham-operation group (group B). Acute cerebral ischemia model was established in group D by photochemical induction. Group D was treated with simple NSCs transplantation after 24 hours of modeling. BDNF pretreated NSCs transplantation group C was treated with the same amount of solvent. 1 day before transplantation, 3 days after transplantation, 7 days after transplantation and 14 days after transplantation for 21 days and 28 days after transplantation, accelerated rotation test and forelimb grip test were performed. On the 28th day after transplantation, 5 mice in group E received immunofluorescence staining of microtubule-associated protein 2- MAP-2 and glial fibrillary acidic protein (GFAP). Results after 3 days of transplantation, 7 days after transplantation, 14 days after transplantation, 21 days after transplantation, 28 days after transplantation, the accelerated rotation time was in turn from group E to group D, group B, P0.05. After 14 days, 21 days and 28 days, the order of forelimb grasping force was E group D group and B group P 0.05. Both Edu/GFAP positive cells and Edu/MAP-2 double positive cells were found in E group 28 days after transplantation. Conclusion NSCs transplantation can promote the recovery of behavioral function after ischemic stroke. After pretreatment with BDNF, the effect is better.
【作者单位】：兰州大学第二医院神经外科;
【基金】：甘肃省高等学校科研项目(No.2014B-001) 甘肃电信萃英科研基金资助项目(No.lzudxcy-2014-8)
【分类号】：R743.3

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