生脉注射液联合去甲肾上腺素对多器官功能障碍综合征大鼠淋巴细胞凋亡及凋亡蛋白的影响

发布时间：2018-02-09 02:01

本文关键词： 多器官功能障碍生脉注射液去甲肾上腺素免疫功能　出处：《中国免疫学杂志》2016年08期 　论文类型：期刊论文

【摘要】：目的:研究生脉注射液联合去甲肾上腺素对多器官功能障碍综合征大鼠细胞免疫的影响。方法:选取40只健康雄性大鼠,通过应用酵母多糖腹腔注射致MODS模型,采用数字表法随机分为对照组、生脉注射液组、去甲肾上腺素组以及生脉注射液联合去甲肾上腺组,每组10只。用药后6、24、48 h检测血清中TNF-α、IL-1β、IL-12和IL-2的浓度,免疫组化检测大鼠脾脏caspase-3、caspase-8、caspase-9活性,流式细胞技术测定淋巴细胞凋亡情况及Th1/Th2。结果:1与对照组相比,生脉注射液组、去甲肾上腺组以及联合用药组,TNF、IL-1β浓度上升不是很明显,且随着治疗时间的延长,治疗各组TNF、IL-1β浓度逐渐下降,联合用药组TNF、IL-1β基本趋于正常;而IL-12、IL-2趋势则相反,治疗各组较对照组血清IL-12、IL-2浓度明显升高,联合用药组升高更加明显。2与对照组相比,生脉注射液组、去甲肾上腺素组以及联合用药组caspase-3、caspase-8、caspase-9表达均显著减少,且联合用药组凋亡蛋白的表达下降更明显,差异有统计学意义(P0.05)。3生脉注射液组、去甲肾上腺素组以及联合用药组淋巴细胞凋亡的阳性细胞数比率较对照组明显减少,联合用药组淋巴细胞凋亡的阳性细胞数减少较生脉注射液组以及去甲肾上腺素组更明显;相比于对照组,生脉注射液组、去甲肾上腺素组以及联合用药组Th1/Th2比率明显增高,联合用药组增高更明显,差异有统计学意义(P0.05)。结论:生脉注射液联合去甲肾上腺素对多器官功能障碍大鼠具有减轻炎症反应和改善免疫功能的作用。
[Abstract]:Objective: to study the effect of Mai injection combined with norepinephrine on cellular immunity of rats with multiple organ dysfunction syndrome. Methods: MODS model was induced by intraperitoneal injection of yeast polysaccharide in 40 healthy male rats. 10 rats in each group were randomly divided into control group, Shengmai injection group, norepinephrine group and Shengmai injection combined with norepinephrine group. The activity of caspase-3, caspase-8 and caspase-9 in spleen, apoptosis of lymphocytes and Th1 / Th2 were detected by flow cytometry. Results compared with control group, the concentration of TNF-IL-1 尾 in Shengmai injection group, norepinephrine group and combined treatment group was not significantly increased. With the prolongation of the treatment time, the concentration of TNFN IL-1 尾 in the treatment group decreased gradually, while in the combined treatment group, the level of TNFN IL 1 尾 tended to be normal, while the trend of IL 12 and IL 2 was opposite, and the concentration of IL 12 and IL 2 in the treatment group was significantly higher than that in the control group. Compared with the control group, the expression of caspase-3, caspase-8, caspase-8 and caspase-9 in Shengmai injection group, norepinephrine group and combined treatment group were significantly decreased, and the expression of apoptotic protein in combination group was more obvious than that in control group. The difference was statistically significant (P 0.05.3). The percentage of apoptotic positive cells in norepinephrine group, norepinephrine group and combination group was significantly lower than that in control group. Compared with the control group, Shengmai injection group, norepinephrine group and combined treatment group, the Th1/Th2 ratio of the combined treatment group was significantly higher than that of the Shengmai injection group, the norepinephrine group and the combined treatment group. Conclusion: Shengmai injection combined with norepinephrine can attenuate inflammation and improve immune function in rats with multiple organ dysfunction.
【作者单位】：北京市昌平区医院重症医学科;北京市朝阳医院外科重症监护一病区;
【分类号】：R459.7

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