胆维丁乳对酵母多糖致多器官功能衰竭小鼠肝脏的作用

发布时间：2018-03-09 04:25

本文选题：胆维丁乳　切入点：酵母多糖　出处：《中国医科大学学报》2017年03期 　论文类型：期刊论文

【摘要】：目的研究胆维丁乳对酵母多糖致多器官功能衰竭小鼠肝脏损伤的作用。方法 C57 BL/6源性野生小鼠36只,随机分为阴性对照(CON)组、胆维丁乳(CCE)组、酵母多糖(Z)组、CCE+Z组,每组各9只。CON组及Z组纯水喂养,CCE组及CCE+Z组应用胆维丁乳(20μL溶于200 mL纯水)避光喂养。14 d后Z组及CCE+Z组酵母多糖(500 mg/kg)腹腔注射。18 h后处死各组小鼠,留肝脏标本行病理组织学检查;检测各组小鼠血清中谷丙转氨酶(ALT)含量;应用Western blotting、实时PCR方法测定各组小鼠肝脏组织白细胞介素6(IL-6)、白细胞介素18(IL-18)表达。结果病理组织学显示,CCE+Z组组织损伤较Z组轻,较对照组重。与CON组比较,血清ALT水平Z组、CCE+Z组升高;而CCE组降低,差异均具有统计学意义(均P0.05)。IL-6、IL-18蛋白与mRNA表达结果显示,与CON组及CCE+Z组比较Z组明显升高,差异具有统计学意义(均P0.05)。结论胆维丁乳对酵母多糖致肝脏损伤能够起到一定的保护作用。
[Abstract]:Objective to study the effect of Danvidine milk on liver injury in mice with multiple organ failure induced by yeast polysaccharides. Methods Thirty-six wild mice of C57 BL/6 origin were randomly divided into three groups: negative control group (Conn), gallitrin group (CCEE) group, yeast polysaccharide group (ZZ) group. Nine rats in each group were fed with pure water in each group. CCE and CCE Z groups were treated with 20 渭 L of cholaventin milk dissolved in 200ml pure water. The mice of group Z and group CCE Z were sacrificed by intraperitoneal injection of yeast polysaccharide (500mg / kg) for 18 h. The liver samples were examined by histopathology, and the serum alanine aminotransferase (alt) content was detected in each group. Western blotting and real-time PCR were used to detect the expression of interleukin-6 and interleukin-18IL-18 in the liver of mice in each group. Results the histopathological results showed that the tissue damage in the CCEZ group was lighter than that in the Z group and was more serious than that in the control group. The level of serum ALT increased in group Z and decreased in group CCE (P 0.05). IL-6 / IL-18 protein and mRNA expression in group Z were significantly higher than those in group Z and CON and CCE Z groups, and the levels of IL-18 in group Z were significantly higher than those in group Z (P 0.05), and the expression of IL-18 in group Z was significantly higher than that in group Z (P 0.05). The difference was statistically significant (P 0.05). Conclusion Dentiverin milk can protect liver injury induced by yeast polysaccharides.
【作者单位】：中国医科大学附属盛京医院临床营养科;中国医科大学公共基础学院计算机教研室;
【基金】：国家自然科学基金(81170065,81570811)
【分类号】：R459.7

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