SpA刺激单核巨噬细胞表达早期致炎因子的实验研究

发布时间：2018-03-09 08:49

本文选题：金黄色葡萄球菌蛋白A　切入点：THP-　出处：《重庆医学》2016年11期 　论文类型：期刊论文

【摘要】：目的探讨金黄色葡萄球菌蛋白A(SpA)对单核巨噬细胞表达早期致炎因子TNF-α、IL-1和IL-6的影响。方法用一定浓度SpA与THP-1细胞培养不同时间,用MTT法测定SpA对THP-1细胞增殖的影响;用ELISA测定培养液中TNF-α、IL-1和IL-6的水平;用RT-PCR检测细胞TNF-α、IL-1和IL-6相应mRNA的表达,并对结果进行统计学分析。结果 SpA对THP-1细胞增殖的影响与其作用剂量有关。SpA可促进巨噬细胞分泌TNF-α、IL-1和IL-6及表达相应mRNA,且呈一定剂量-效应和时间-效应关系。SpA刺激巨噬细胞分泌TNF-α、IL-1、IL-6和表达相应mRNA均在12h达高峰。SpA刺激组TNF-α、IL-1和IL-6的表达和释放均显著升高(P0.01)。结论 SpA可明显促进单核巨噬细胞表达和分泌早期致炎细胞因子TNF-α、IL-1和IL-6。SpA在启动金葡菌性脓毒症及促进其发展中,具有不可忽视的作用。
[Abstract]:Objective to investigate the effect of staphylococcus aureus protein Agni Spa on the expression of early inflammatory factor TNF- 伪 IL-1 and IL-6 in mononuclear macrophages. Methods the effects of SpA on the proliferation of THP-1 cells were measured by MTT assay after cultured with SpA and THP-1 cells for different time. The levels of TNF- 伪 -1 and IL-6 in culture medium were measured by ELISA, and the expression of TNF- 伪 -1 and IL-6 mRNA were detected by RT-PCR. Results the effect of SpA on the proliferation of THP-1 cells was related to the dose of THP-1 cells. Spa could promote macrophages to secrete TNF- 伪 -1 and IL-6 and express the corresponding mRNAs, and showed a dose-effect and time-effect relationship. Spa. The expression and release of IL-1 and IL-6 in macrophages reached their peak at 12h. Conclusion SpA can significantly promote the expression and secretion of TNF- 伪 IL-1 and IL-1 and IL-1 and IL-1- 伪 in mononuclear macrophages and the secretion of early inflammatory cytokines TNF- 伪 IL-1 and IL-1- 伪 in mononuclear macrophages. IL-6.SpA is involved in the initiation and development of Staphylococcus aureus sepsis. It can't be ignored.
【作者单位】：遵义医学院第三附属医院重症医学科;遵义医学院;遵义医学院微生物教研室;
【基金】：贵州省大学生创新课题(201413653004)
【分类号】：R459.7

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