脑红蛋白在感染性休克早期大鼠脑组织中的动态变化

发布时间：2018-03-10 05:13

本文选题：感染性休克　切入点：脑红蛋白　出处：《临床麻醉学杂志》2016年12期 　论文类型：期刊论文

【摘要】：目的探讨感染性休克早期大鼠脑组织中脑红蛋白(neuroglobin,NGB)含量的动态变化在感染性休克中可能发挥的作用。方法健康雄性SD大鼠60只,12~15周龄,体重300~350g,随机分为两组,每组30只。M组经尾静脉注射脂多糖(LPS)10mg/kg制备大鼠感染性休克模型,当SBP下降幅度达到基础值的40%时认为感染性休克模型复制成功;C组给予等量生理盐水。分别在给LPS前(T_0)、给LPS后1h(T_1)、2h(T_2)、4h(T_3)、6h(T_4)采用ELISA法检测血清中TNF-α、IL-6浓度和NGB含量,Western blot法检测脑组织中NGB、半胱天冬酶-3(caspase-3)的含量。结果与C组比较,T1~T4时M组血清中的TNF-α、IL-6浓度明显增高(P0.05),脑组织中caspase-3含量明显增加(P0.05);T_3、T_4时血清和脑组织中NGB含量明显增高(P0.05)。结论感染性休克后大鼠脑组织发生了炎症损伤、细胞凋亡病理学改变,脑红蛋白在感染性休克所致的损伤脑组织中含量增加,推测其可能发挥了内源性神经保护功能。
[Abstract]:Objective to investigate the role of the dynamic changes of brain erythroprotein neuroglobinus NGB (NGB) in the early stage of septic shock in rats. Methods 60 healthy male Sprague-Dawley rats were randomly divided into two groups. The septic shock model of rats in group M was induced by injecting lipopolysaccharide (LPS) 10 mg / kg into tail vein of 30 rats in each group. When the decrease of SBP reached the base value, it was considered that the septic shock model was successfully replicated and group C was given the same amount of normal saline. Before LPS was given, T0 was given, and 1 hour after LPS was given, respectively, 2hstr T2T 2hStaph, 4hStaph T3hT 3n, 6hStam T4). ELISA method was used to detect TNF- 伪 -IL-6 and NGB content. Western blot method was used to detect the concentration of TNF- 伪, IL-6 and NGB. Results compared with group C, the concentration of TNF- 伪 and IL-6 in serum of group M was significantly higher than that in group C (P 0.05), and the content of caspase-3 in brain tissue was significantly higher than that in group C. Conclusion the content of NGB in serum and brain tissue is significantly higher than that in group C. Conclusion the content of NGB in serum and brain tissue is significantly higher than that in group C. Conclusion the level of NGB in serum and brain tissue of group M is significantly higher than that of group C. Conclusion the level of NGB in serum and brain tissue of group M is significantly higher than that of group C. Inflammatory injury occurred in the brain of the rats after sexual shock. The pathological changes of apoptosis and the increase of the content of brain erythroprotein in the injured brain tissue caused by septic shock suggest that it may play an endogenous neuroprotective function.
【作者单位】：解放军107医院麻醉科;滨州医学院烟台附院麻醉科;
【分类号】：R459.7

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