迷走神经电刺激对内毒素血症小鼠肠道上皮细胞紧密连接及肺的血气屏障的影响

发布时间：2018-03-16 07:18

  本文选题：内毒素血症　切入点：迷走神经电刺激　出处：《广东医学》2017年16期 　论文类型：期刊论文

【摘要】：目的探讨迷走神经电刺激活化胆碱能抗炎通路对内毒素血症小鼠的肠道上皮细胞紧密连接及肺的血气屏障的保护作用及其可能机制。方法雄性Balb/c小鼠腹腔注射脂多糖10 mg/kg,复制内毒素血症模型。将小鼠随机分为4组:(1)假手术组,暴露分离迷走神经但不给予电刺激,给予生理盐水0.25 m L腹腔注射;(2)内毒素血症组,暴露分离迷走神经但不给予电刺激,给予腹腔注射脂多糖10 mg/kg;(3)迷走神经电刺激组,在脂多糖腹腔注射后,用5%的戊巴比妥钠腹腔注射麻醉,暴露分离右侧迷走神经,连接迷走神经电刺激仪刺激右侧迷走神经10 min;(4)α-银环蛇毒素(α-BGT)组,在迷走神经电刺激前20 min皮下给予α-BGT 0.5μg/只,余操作同迷走神经电刺激组。在给予脂多糖腹腔注射12 h后采集小鼠的回肠及肺组织标本。结果内毒素血症组、α-BGT组右旋糖酐(FITC-Dextran)水平均高于假手术组,差异有统计学意义(P0.05)。内毒素血症组、α-BGT组FITC-Dextran水平均高于迷走神经电刺激组,差异有统计学意义(P0.05)。内毒素血症组肺组织occludin水平低于假手术组,肠道组织occludin水平低于假手术组、NF-κB水平高于假手术组(P0.05);α-BGT组occludin水平低于假手术组,NF-κB水平高于假手术组(P0.05),肠道组织occludin水平低于假手术组、NF-κB水平高于假手术组(P0.05);迷走神经电刺激组肺组织occludin水平高于内毒素血症组和α-BGT组,NF-κB水平低于LPS组和α-BGT组,肠道组织occludin水平高于内毒素血症组和α-BGT组,NF-κB水平低于LPS组和α-BGT组(P0.05)。结论迷走神经电刺激活化胆碱能抗炎通路对内毒素血症小鼠肠道上皮和肺的血气屏障的通透性的保护是通过烟碱型乙酰胆碱受体α7亚基及NF-κB和MLCK通路来发挥作用的。
[Abstract]:Objective to investigate the protective effect of vagal electrical stimulation activated cholinergic anti-inflammatory pathway on tight junction of intestinal epithelial cells and blood gas barrier of lung in endotoxemia mice and its possible mechanism. Methods male Balb/c mice were injected with lipid intraperitoneally. The model of endotoxemia was established by 10 mg / kg polysaccharide. The mice were randomly divided into 4 groups: 1) sham-operated group. The vagus nerve was exposed to isolated vagus nerve but not stimulated by electric stimulation. The rats were given normal saline 0.25 mL intraperitoneal injection of Pao 2) endotoxemia group, exposure to isolated vagus nerve but not electric stimulation, and intraperitoneal injection of lipopolysaccharide 10 mg / kg 3) vagus nerve stimulation group. After intraperitoneal injection of lipopolysaccharide (LPS), 5% pentobarbital sodium was injected intraperitoneally to anesthetize the right vagus nerve. The right vagus nerve was separated from the right vagus nerve, and the right vagus nerve was stimulated by the vagus nerve electrical stimulator for 10 minutes. Subcutaneously, 伪 -BGT 0.5 渭 g / mouse was given 20 min before vagus nerve stimulation. After 12 hours of intraperitoneal injection of lipopolysaccharide, the ileum and lung tissues of mice were collected. Results the levels of FITC-Dextranan in endotoxemia group and 伪 -BGT group were higher than those in sham operation group. The level of FITC-Dextran in endotoxemia group and 伪 -BGT group was higher than that in vagal nerve stimulation group, and the difference was statistically significant (P 0.05). The level of occludin in endotoxemia group was lower than that in sham operation group. The level of occludin in intestinal tissue was lower than that in sham operation group (P 0.05), the level of occludin in 伪 -BGT group was lower than that in sham operation group (P 0.05), the level of occludin in intestinal tissue was lower than that in sham operation group (P 0.05), and the level of vagus nerve was higher in intestinal tissue than that in sham operation group (P 0.05). The level of occludin in lung tissue of electrical stimulation group was higher than that of endotoxemia group and 伪 -BGT group, and the level of NF- 魏 B was lower in electric stimulation group than that in LPS group and 伪 -BGT group. The level of occludin in intestinal tissue was higher than that in endotoxemia group and 伪 -BGT group, and the level of NF- 魏 B in intestinal tissue was lower than that in LPS group and 伪 -BGT group. Conclusion the permeability of blood gas barrier of intestinal epithelium and lung in mice with endotoxemia by vagal nerve stimulation activated cholinergic anti-inflammatory pathway is lower than that in LPS group and 伪 -BGT group. The protection is mediated by nicotinic acetylcholine receptor 伪 7 subunit and NF- 魏 B and MLCK pathways.
【作者单位】： 河南大学淮河医院急诊科;河南大学淮河医院神经内科;河南大学淮河医院呼吸科;
【基金】：河南省教育厅科学技术研究重点项目(编号:16A320067)
【分类号】：R459.7
，

本文编号：1618880