Toll样受体4在百草枯中毒急性肺损伤中的作用

发布时间：2018-03-28 02:15

本文选题：百草枯　切入点：中毒　出处：《中国医科大学学报》2017年01期

【摘要】：目的探讨Toll样受体4(TLR4)在百草枯(PQ)中毒急性肺损伤中的作用及相关机制。方法野生小鼠(C57BL/6J)和TLR4基因敲除小鼠(C57BL/10Sc N)各20只,随机分成野生小鼠对照(WT)组、WT+PQ组,TLR4基因敲除小鼠对照(TLR4-ko)组、TLR4-ko+PQ组,每组10只。腹腔注射PQ,24 h后处死小鼠,观察各组小鼠肺脏病理改变(HE染色和电镜)、血气分析结果、肺脏TLR4和炎症介质的表达情况。结果腹腔注射PQ 24 h后野生小鼠出现明显急性肺损伤表现,包括血气氧分压明显下降、肺脏损伤急性期病理改变、TLR4表达明显升高(伴随肺脏TNF-α、IL-1β和NF-κB p65表达明显升高);而TLR4基因敲除小鼠在接触PQ后肺损伤情况明显减轻,WT+PQ组和TLR4-ko+PQ组比较差异具有统计学意义(P0.05)。结论 TLR4是PQ中毒急性肺损伤的重要介质,阻断TLR4信号通路对PQ中毒所致肺损伤具有保护作用。
[Abstract]:Objective to investigate the role of Toll like receptor 4 (TLR4) in acute lung injury induced by paraquat (PQ). Methods 20 wild mice (C57BL / 6J) and 20 TLR4 gene knockout mice (C57BL / 10Sc N) were used. The mice were randomly divided into WT PQ group and TLR4 gene knockout control TLR4-koo group (10 mice in each group). The mice were killed 24 hours after intraperitoneal injection of PQ4. The pathological changes of lung were observed by HE staining, electron microscopy and blood gas analysis. Results 24 h after intraperitoneal injection of PQ, the expression of TLR4 and inflammatory mediators in the lungs of wild mice showed obvious acute lung injury, including the decrease of partial pressure of oxygen in blood. The expression of TLR4 was significantly increased in the acute stage of lung injury (accompanied by a marked increase in the expression of TNF- 伪 IL-1 尾 and NF- 魏 B p65 in the lungs), while the lung injury in TLR4 knockout mice was significantly reduced after exposure to PQ. There was a significant difference between WT PQ group and TLR4-ko PQ group. Conclusion TLR4 is an important medium for acute lung injury caused by PQ poisoning. Blocking TLR4 signaling pathway has protective effect on lung injury induced by PQ poisoning.
【作者单位】：中国医科大学附属第一医院急诊科;
【基金】：国家自然科学基金(81571882) 辽宁省科学技术计划(2013225303) 沈阳市科技计划(F16-206-9-02)
【分类号】：R595.4;R563

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