血管内皮细胞微粒在脓毒症大鼠血管渗漏中的作用

发布时间：2018-04-03 01:19

本文选题：脓毒症　切入点：微粒　出处：《第三军医大学学报》2016年08期

【摘要】：目的探讨血管内皮细胞微粒在脓毒症大鼠血管渗漏中的作用。方法采用盲肠结扎穿孔术(cecal ligation and puncture,CLP)复制大鼠脓毒症模型,用流式细胞术及透射电镜对微粒进行鉴定,观察脓毒症大鼠血管渗漏变化与血液中微粒之间的关系;检测脓毒症大鼠来源微粒和LPS刺激肺静脉内皮细胞、血小板和中性粒细胞产生的微粒对正常大鼠和单层肺静脉内皮细胞通透性的影响及与紧密连接的关系。结果脓毒症大鼠肺、肾、肠血管通透性及血液中微粒含量均随时间显著增加(P0.05);脓毒症大鼠来源微粒可显著增加正常大鼠肺、肾、肠血管通透性(P0.01)。LPS刺激血管内皮细胞产生的微粒可以显著增加正常大鼠肺血管通透性(P0.05),而LPS刺激血小板和中性粒细胞产生的微粒对正常大鼠肺血管通透性无明显作用。LPS刺激血管内皮细胞产生的微粒也可明显降低单层肺静脉内皮细胞跨膜电阻(P0.01),降低肺静脉内皮细胞紧密连接ZO-1表达(P0.05)。结论内皮细胞微粒可以参与脓毒症大鼠血管渗漏的调节,其机制可能与影响血管内皮细胞间紧密连接有关。
[Abstract]:Objective to investigate the role of vascular endothelial cell particles in vascular leakage in septic rats.Methods the sepsis model was established by cecal ligation and puncturing in rats. The microparticles were identified by flow cytometry and transmission electron microscopy. The relationship between blood vessel leakage and blood particles in septic rats was observed.The effects of particulate matter derived from sepsis and LPS stimulation on the permeability of endothelial cells of pulmonary vein, platelets and neutrophils were measured in normal and monolayer pulmonary vein endothelial cells.Results the blood vessel permeability and the content of blood particles in the lungs, kidneys and intestines of sepsis rats were significantly increased with time, while the particles derived from sepsis rats could significantly increase the lungs and kidneys of normal rats.P0.01n. LPS-stimulated microparticles produced by vascular endothelial cells significantly increased pulmonary vascular permeability in normal rats, while LPS stimulated platelet and neutrophil particles did not significantly contribute to pulmonary vascular permeability in normal rats.LPS-stimulated microparticles of vascular endothelial cells also significantly decreased the transmembrane resistance of monolayer pulmonary vein endothelial cells (P0.01A), and the expression of ZO-1 tightly connected to pulmonary vein endothelial cells (P0.05).Conclusion Endothelial microparticles may be involved in the regulation of vascular leakage in septic rats, and its mechanism may be related to the influence of tight junction between vascular endothelial cells.
【作者单位】：第三军医大学大坪医院野战外科研究所第二研究室创伤、烧伤与复合伤国家重点实验室;
【基金】：国家自然科学基金面上项目(81570441)~~
【分类号】：R459.7

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