盐酸戊乙奎醚上调β抑制蛋白-1与M3受体的关系研究

发布时间：2018-04-18 06:46

本文选题：M受体 + 盐酸戊乙奎醚　；参考：《重庆医学》2017年13期

【摘要】：目的探讨在脂多糖(LPS)致肺微血管内皮损伤中盐酸戊乙奎醚(PHC)上调β抑制蛋白-1(β-arrestin-1)的作用与M3受体的关系。方法 M3shRNA转染肺微血管内皮细胞和正常肺微血管内皮细胞,分为LPS组(A)、盐酸戊乙奎醚+LPS组(B)、LPS+M3shRNA转染组(C)和盐酸戊乙奎醚+LPS+M3shRNA转染组(D),激光共聚焦观察细胞骨架变化,检测细胞上清液中乳酸脱氢酶(LDH)水平,免疫荧光化学法检测血管内皮细胞黏附分子-1(VCAM-1)蛋白表达,Western blot法和实时定量PCR法检测β-arrestin-1表达。结果与A组、C组比较,B组、D组肌动蛋白骨架排列整齐,LDH水平和VCAM-1蛋白表达降低,β-arrestin-1表达升高;与A组、B组相比较,C组、D组肌动蛋白骨架排列整齐,LDH水平和VCAM-1蛋白表达降低,β-arrestin-1表达无明显改变。结论沉默M3受体有助于降低脂多糖诱导的肺微血管内皮细胞损伤。但盐酸戊乙奎醚上调β-arrestin-1的作用与M3受体是否存在无必然联系。
[Abstract]:Objective to investigate the relationship between the up-regulation of 尾 -arrestin-1 (尾 -arrestin-1) and M3 receptor in pulmonary microvascular endothelial injury induced by lipopolysaccharide (LPS).Methods Pulmonary microvascular endothelial cells and normal pulmonary microvascular endothelial cells were transfected with M3shRNA. The cells were divided into LPS group, LPS group and LPS M3shRNA group. The cytoskeleton was observed by confocal laser.The level of lactate dehydrogenase (LDH) in supernatant was detected. The expression of vascular endothelial cell adhesion molecule-1 (VCAM-1) protein was detected by immunofluorescence method. The expression of 尾 -arrestin-1 was detected by Western blot and real-time quantitative PCR.Results compared with group A and C, the level of VCAM-1 and the expression of 尾 -arrestin-1 were decreased and 尾 -arrestin-1 increased in group B and D.Compared with group A and B, the level of VCAM-1 and the expression of VCAM-1 protein in group C and D were decreased, but the expression of 尾 -arrestin-1 was not changed.Conclusion silencing M 3 receptor can reduce the injury of pulmonary microvascular endothelial cells induced by lipopolysaccharide.However, the up-regulation of 尾 -arrestin-1 by pentenehyclidine hydrochloride was not associated with the existence of M _ 3 receptor.
【作者单位】：武汉大学中南医院麻醉科;
【基金】：国家自然科学基金青年基金项目(81101408) 湖北省武汉市科技局晨光计划项目(2016070204010150)
【分类号】：R459.7

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