磨痂术对烫伤大鼠术后创面铜绿假单胞菌感染的干预作用研究

发布时间：2018-04-26 12:58

本文选题：磨痂术 + 深Ⅱ度烫伤　；参考：《中华医院感染学杂志》2017年22期

【摘要】：目的探讨磨痂术对烫伤大鼠术后创面铜绿假单胞菌感染的干预作用。方法 48只雄性Wistar大鼠随机分为A组和B组,每组24只,A组大鼠制造烫伤模型1~2小时后对创面实施磨痂术,术后涂铜绿假单胞菌标准菌株;B组大鼠制造烫伤模型1~2小时后对创面实施削痂术,术后涂铜绿假单胞菌标准菌株;两组实验动物于伤后3、5、7天各随机处死大鼠8只,左心室采血进行细菌培养,取创面组织标本进行HE染色及组织匀浆细菌定量培养。结果 A组血培养铜绿假单胞菌总阳性率明显低于B组,经比较差异有统计学意义(P0.05);组织匀浆细菌定量培养统计结果显示,A组细菌计数明显少于B组,经比较差异有统计学意义(P0.01);HE染色结果可见,两组随着受伤时间的延长,炎症程度明显加重,B组炎症程度重于A组,A组创伤组织侵袭性感染率明显少于B组,经比较差异有统计学意义(P0.05)。结论磨痂术是早期处理烫伤深Ⅱ度创面的一种更为合理的手术方式。
[Abstract]:Objective to investigate the effect of scab grinding on Pseudomonas aeruginosa infection after scalding in rats. Methods 48 male Wistar rats were randomly divided into two groups: group A (n = 24) and group B (n = 24). Two hours after scald injury, scab removal was performed on the wound surface of rats in group B, and 8 rats were killed at random in each group on the 3rd and 7th day after injury. The left ventricular blood was collected for bacterial culture, the wound tissue samples were stained with HE and the tissue homogenate bacteria were cultured quantitatively. Results the total positive rate of Pseudomonas aeruginosa in blood culture in group A was significantly lower than that in group B (P 0.05), and the count of bacteria in group A was significantly lower than that in group B. The results of HE staining showed that the degree of inflammation in group B was significantly worse than that in group A, and the invasive infection rate of trauma tissue in group A was significantly lower than that in group B. The difference was statistically significant (P 0.05). Conclusion escharectomy is a more reasonable method for early treatment of deep second degree scald wound.
【作者单位】：济南市中心医院烧伤科;
【分类号】：R644

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