烧伤病房金黄色葡萄球菌分离株的分子分型及其耐药性

发布时间：2018-04-30 13:19

本文选题：烧伤 + 葡萄球菌　；参考：《中国组织工程研究》2016年37期

【摘要】：背景:至20世纪80年代,耐甲氧西林金黄色葡萄球菌感染已经遍布全球,目前已成为院内感染的首要病原菌。目的:了解烧伤病房金黄色葡萄球菌的分子型别及其对临床常用抗生素的耐药情况,为防控耐甲氧西林金黄色葡萄球菌感染提供依据。方法:收集解放军兰州军区乌鲁木齐总医院烧伤病房53株金黄色葡萄球菌,运用PCR方法以及头孢西丁实验鉴定耐甲氧西林金黄色葡萄球菌菌株,并且利用spa分型、SCCmec分型、MLST分型方法对耐甲氧西林金黄色葡萄球菌进行分子分型,同时测定所有金黄色葡萄球菌对苯唑西林等17种药物的敏感性,分析不同型别金黄色葡萄球菌特别是耐甲氧西林金黄色葡萄球菌的耐药情况。结果与结论:(1)53株金黄色葡萄球菌中有41株检测到meoA基因,另有2株头孢西丁实验阳性,均定义为耐甲氧西林金黄色葡萄球菌,这些耐甲氧西林金黄色葡萄球菌可分为3种SCCmec型,4种spa型,3种ST型。(2)优势耐甲氧西林金黄色葡萄球菌克隆为ST239-III-t030占90.7%,该克隆均对苯唑西林等10种被检抗生素高度耐药。(3)结果说明,该院烧伤病房耐甲氧西林金黄色葡萄球菌分离率较高,MRSA-ST239-III-t030占据绝对优势,具有高度耐药性,呈现爆发流行特征。
[Abstract]:Background: by the 1980s, methicillin-resistant Staphylococcus aureus infection has spread all over the world and has become the leading pathogen of nosocomial infection. Objective: to investigate the molecular types of Staphylococcus aureus and its resistance to antibiotics in burn ward, and to provide evidence for the prevention and control of methicillin-resistant Staphylococcus aureus infection. Methods: 53 strains of Staphylococcus aureus were collected from burn ward of Urumqi General Hospital of Lanzhou military region of PLA. The strains resistant to methicillin-resistant Staphylococcus aureus were identified by PCR method and cefoxitin test. The molecular typing of methicillin-resistant Staphylococcus aureus was carried out by using spa typing method. The sensitivity of all Staphylococcus aureus to oxacillin and other 17 drugs was determined. To analyze the drug resistance of different types of Staphylococcus aureus, especially methicillin-resistant Staphylococcus aureus. Results and conclusion meoA gene was detected in 41 of 53 strains of Staphylococcus aureus and 2 strains were positive for cefoxitin, which were defined as methicillin-resistant Staphylococcus aureus. These methicillin-resistant Staphylococcus aureus can be divided into three SCCmec types, four spa types and three St types.) the dominant methicillin-resistant Staphylococcus aureus was cloned into 90.7% of the methicillin-resistant Staphylococcus aureus, and the clones were highly resistant to oxacillin and other 10 tested antibiotics. The isolation rate of methicillin-resistant Staphylococcus aureus in burn ward of our hospital was higher than that of MRSA-ST239-III-t030.
【作者单位】：解放军第二七三医院传染结核科;解放军兰州军区乌鲁木齐总医院检验科;
【基金】：国家自然科学基金(81401703)~~
【分类号】：R644

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