参附注射液联合去甲肾上腺素治疗脓毒症休克大鼠与其对血红蛋白清道夫受体影响的研究

发布时间：2018-05-01 09:36

本文选题：参附注射液 + 脓毒症　；参考：《中华医院感染学杂志》2016年04期

【摘要】：目的探讨参附注射液联合去甲肾上腺素对脓毒症休克大鼠的影响及其相关的分子机制,为临床早期诊断脓毒症及预后评估提供依据。方法选取60只SPF级健康SD大鼠,采用随机数字表法分为正常组(A组)、模型组(B组)、西药组(C组)、参附组(D组)、参附联合西药组(E组),每组各12只,采用对股静脉连续滴注脂多糖(LPS)进行造模,A组不造模,静脉注射生理盐水;B组造模,静脉注射生理盐水;C组造模,静脉注射重酒石酸去甲肾上腺素注射液;D组造模,静脉注射参附注射液;E组造模,静脉注射参附注射液和重酒石酸去甲肾上腺素注射液,酶联免疫(ELISA)法检测单用西药和参附注射液以及中西药合用治疗前后血液中血红蛋白清道夫受体(sCD163)、C-反应蛋白(CRP)、降钙素原(PCT)的含量。结果 E组大鼠一般情况优于B组,E组大鼠sCD163、CRP、PCT的含量明显低于C、D组,组间比较差异有统计学意义(P0.05)。结论参附注射液能通过抑制sCD163、CRP、PCT的表达改善大鼠脓毒症休克症状,sCD163可以作为脓毒症休克临床中药疗效观察的敏感指标。
[Abstract]:Objective to investigate the effect of Shenfu injection combined with norepinephrine on septic shock rats and its molecular mechanism, and to provide evidence for early clinical diagnosis of sepsis and evaluation of prognosis. Methods Sixty SPF healthy SD rats were randomly divided into normal group A group, model group B group, western medicine group C group, Shenfu group D group, Shenfu combined group E group with 12 rats in each group. The models of group A, group B, group C, and group D, respectively, were established by continuous infusion of lipopolysaccharide (LPSs) into femoral vein, and those of group A were not made by intravenous injection of noradrenaline tartrate, and those of group B were made by intravenous injection of normal saline, and those of group D were made by intravenous injection of noradrenaline tartrate. Group E was injected with Shenfu injection, Shenfu injection and norepinephrine heavy tartrate injection were injected intravenously. The levels of hemoglobin scavenger receptor (sCD163), C-reactive protein (CRPX) and procalcitonin (PCT) in blood before and after treatment with western medicine, Shenfu injection and traditional Chinese medicine were detected by Elisa. Results the general situation of group E was better than that of group B (P 0.05). Conclusion Shenfu injection can improve the symptoms of septic shock by inhibiting the expression of sCD163 CRPnPCT in rats. It can be used as a sensitive index to observe the clinical efficacy of traditional Chinese medicine in septic shock.
【作者单位】：武汉市中西医结合医院武汉市第一医院急诊科;
【基金】：武汉市卫计委科研基金资助项目(WZ14A05)
【分类号】：R459.7

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