雌激素在脓毒症大鼠骨骼肌蛋白高分解代谢中的作用及其可能机制研究

发布时间：2018-05-12 14:51

本文选题：雌激素 + 脓毒症　；参考：《肠外与肠内营养》2016年03期

【摘要】：目的:探讨雌激素在脓毒症大鼠骨骼肌蛋白高分解代谢中的作用及其可能机制。方法:采用腹腔内注射10 mg/kg内毒素制造脓毒症动物模型。将36只大鼠随机分为正常对照组、脓毒症组、脓毒症+雌激素注射组,每组12只。采用高效液相色谱分析法测定酪氨酸和三甲基组氨酸,实时聚合酶链式反应(RT-PCR)分析肌肉和下丘脑组织中白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)、骨骼肌萎缩基因(MuRF-1)、雌激素受体α(ER-α)和雌激素受体β(ER-β)mRNA的表达。结果:脓毒症组大鼠三甲基组氨酸(3-MH)、酪氨酸(Tyr)水平和萎缩基因MuRF-1 mRNA表达上调(P0.05),而且中枢IL-1βmRNA和TNF-αmRNA表达量增加(P0.05),ER-α、ER-βmRNA表达量减少(P0.05)。给予皮下注射雌激素后,外周骨骼肌消耗及中枢炎症减轻,且有显著性差异(P0.05)。下丘脑中雌激素受体表达与炎性递质表达呈负相关(P0.05)。结论:雌激素能缓解骨骼肌高分解代谢,可能与其能减轻下丘脑炎症有关。
[Abstract]:Aim: to investigate the role of estrogen in hypercatabolism of skeletal muscle protein in septic rats and its possible mechanism. Methods: sepsis animal model was established by intraperitoneal injection of endotoxin for 10 mg/kg. Thirty-six rats were randomly divided into normal control group (n = 12) and estrogen injection group (n = 12). Tyrosine and trimethylhistidine were determined by HPLC. Real time polymerase chain reaction (RT-PCR) was used to analyze the expression of IL-1 尾, TNF- 伪, MuRF-1, 伪 -ER- 伪 and 尾 -ER- 尾 in muscle and hypothalamus. Results: the levels of trimethylhistidine 3-MHH (tyrosine After subcutaneous injection of estrogen, the consumption of peripheral skeletal muscle and central inflammation were alleviated, and the difference was significant (P 0.05). The expression of estrogen receptor was negatively correlated with the expression of inflammatory transmitters in hypothalamus (P 0.05). Conclusion: estrogen can relieve the hypercatabolism of skeletal muscle, which may be related to the reduction of hypothalamic inflammation.
【作者单位】：南京大学医学院附属金陵医院(南京军区南京总医院)解放军普通外科研究所;
【分类号】：R459.7

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