Ghrelin对急性心肌梗死大鼠心功能的影响及其机制研究

发布时间：2018-05-17 06:08

本文选题：Ghrelin + 急性心肌梗死　；参考：《重庆医科大学》2013年硕士论文

【摘要】：目的探讨Ghrelin对急性心肌梗死(AMI)大鼠心功能的影响及其可能机制。方法成年雄性SD大鼠18只，结扎冠状动脉前降支建立AMI模型。于建模后第4天，将存活的14只大鼠随机分为模型组(AMI+NS组，，N=7)和处理组(AMI+Ghrelin组，N=7)，处理组按100μg/kg皮下注射Ghrelin，2次/d，间隔12h，共2周，模型组按100μg/kg皮下注射生理盐水(NS)。另设假手术组(N=8)。2周后，超声心动图(UCG)检测大鼠心功能，ELISA法检测血清LDH、CK-MB含量，HE染色观察心肌组织形态学变化，Western blotting检测内质网应激(ERS)标志物GRP-78、CHOP蛋白表达。结果成功建立AMI大鼠模型。与假手术组比较，UCG提示模型组大鼠心功能明显下降(P 0.05)，HE染色可见组织疏松、水肿较明显，血清LDH、CK-MB明显升高(P 0.05)，Western blotting检测显示GRP-78、CHOP蛋白表达增加(P0.05)；与模型组比较，处理组心功能明显改善，组织疏松、水肿明显减轻，血清LDH、CK-MB降低(P0.05)，GRP-78、CHOP蛋白表达下降(P0.05)。结论Ghrelin可能通过抑制ERS途径改善AMI引起的心肌损伤，从而改善心功能。
[Abstract]:Objective to investigate the effect of Ghrelin on cardiac function in acute myocardial infarction (AMI) rats and its possible mechanism. Methods AMI model was established in 18 adult male SD rats by ligation of anterior descending coronary artery. On the 4th day after modeling, 14 surviving rats were randomly divided into two groups: the model group (n = 14) and the treatment group (n = 10). The treatment group was subcutaneously injected with ghrelin (100 渭 g/kg) twice a day for 12 weeks, and the model group was subcutaneously injected with 100 渭 g/kg normal saline. After 8.2 weeks in sham-operation group, the cardiac function of rats was detected by echocardiography (UCGG). The content of serum LDH- CK-MB was detected by Elisa. The morphological changes of myocardium were observed by HE staining. The expression of GRP-78 / CHOP was detected by Western blotting. Results AMI rat model was successfully established. Compared with the sham-operated group, UCG showed that the cardiac function of the model group was obviously decreased P 0.05 and HE staining showed that the tissue was loose, the edema was obvious, and the serum LDH- CK-MB was significantly higher than that of the sham operation group. Western blotting analysis showed that the expression of GRP-78 Chop protein was increased in the model group, and compared with the model group, the expression of GRP-78 Chop protein was increased in the model group. In the treatment group, the cardiac function was improved, the tissue was loose and edema was alleviated, and the expression of GRP-78 chop protein was decreased in serum LDH- CK-MB. Conclusion Ghrelin may improve cardiac function by inhibiting ERS pathway to improve myocardial injury induced by AMI.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R542.22

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