受体CX3CR1基因敲除对同种异系小鼠气管移植术后排斥反应的影响

发布时间：2018-05-21 11:30

本文选题：CXCR + 基因敲除　；参考：《山东医药》2017年40期

【摘要】：目的探讨趋化因子受体CX3CR1基因敲除对同种异系小鼠气管移植后排斥反应的影响。方法将24只小鼠分为同系移植对照组、异系移植对照组、基因敲除移植组,每组各4对。基因敲除移植组、同系移植对照组受体和供体均为BABL/c小鼠;异系移植对照组供体为BABL/c小鼠,受体为C57BL/6小鼠;基因敲除移植组供体为BABL/c小鼠,受体为CX3CR1基因敲除小鼠。各组均行颈背部气管移植,每只受鼠移植2段气管。4周后取出各受鼠移植气管段,石蜡包埋,切片,HE染色,镜下观察管腔纤维化阻塞情况;采用ELISA法检测受鼠血清高迁移率族蛋白1(HMGB1)水平。结果同系移植对照组8个移植气管段均无管腔阻塞,阻塞分数为0;异系移植对照8个移植气管段均出现管腔阻塞,移植气管管腔内充满黏液,镜下见管腔内有大量增生的成纤维细胞,阻塞分数为8.0;受体敲除移植组8个移植气管段中1个出现管腔受挤压、扭曲,1个阻塞不明显,其余6个均未出现较明显的管腔纤维化阻塞,移植气管管腔内充满黏液,镜下见管腔内有大量增生的成纤维细胞,阻塞分数为0.96。移植后4周同系移植对照组、异系移植对照组、基因敲除移植组血清HMGB1水平分别为(29.8±1.6)、(83.6±1.4)、(19.8±0.9)ng/mL,基因敲除移植组明显低于异系移植对照组(P0.05),但与同系移植对照组比较,P0.05。结论受体CX3CR1基因敲除可明显减轻气管移植后排斥反应。
[Abstract]:Objective to investigate the effect of chemokine receptor (CX3CR1) knockout on rejection after tracheal transplantation in allogeneic mice. Methods 24 mice were divided into three groups: homologous transplantation control group, allogeneic transplantation control group and gene knockout transplantation group with 4 pairs in each group. In the gene knockout transplantation group, the recipient and donor of the homologous transplantation control group were both BABL/c mice; the allograft control group was BABL/c mice and the receptor was C57BL/6 mice; the donor of gene knockout transplantation group was BABL/c mice and the receptor was CX3CR1 gene knockout mice. Each group received cervical and dorsal trachea transplantation. Each rat received 2 segments of trachea for 4 weeks. The transplanted trachea segment was embedded in paraffin wax and stained with HE. The obstruction of lumen fibrosis was observed under microscope. The serum high mobility group protein (HMGB1) was detected by ELISA. Results there was no lumen obstruction in 8 tracheal segments in the same transplantation control group, the obstruction fraction was 0 in the allograft control group, and the lumen obstruction was found in all the 8 transplanted tracheal segments in the allograft control group, and the lumen of the transplanted trachea was filled with mucus. Under microscope, a large number of proliferative fibroblasts were found in the lumen, and the blocking fraction was 8.0. In the receptor knockout group, one of the 8 segments of the transplanted trachea was squeezed, distorted, and the obstruction was not obvious. There was no obvious obstruction of fibrosis in the other 6 cases, and the lumen of transplanted trachea was filled with mucus. Under the microscope, a large number of proliferative fibroblasts were found in the lumen with a blocking fraction of 0.96. 4 weeks after transplantation, the serum HMGB1 levels of the same line transplantation control group and the allogeneic transplantation control group were 29.8 卤1.6 卤1.4 ng / mL, respectively. The level of serum HMGB1 in the gene knockout group was significantly lower than that in the allogeneic transplantation control group (P 0.05), but it was significantly lower than that in the same line transplantation control group (P 0.05). Conclusion knockout of receptor CX3CR1 gene can significantly reduce rejection after trachea transplantation.
【作者单位】：安徽省立医院;安徽中医药大学第一附属医院;
【基金】：国家自然科学基金资助项目(30972779)
【分类号】：R655.3

【相似文献】