右侧星状神经节阻滞对体外循环大鼠海马caspase-3表达的影响

发布时间：2018-05-26 18:35

  本文选题：星状神经节阻滞 + 体外循环　； 参考：《广西医科大学》2014年硕士论文

【摘要】：目的：建立大鼠右侧星状神经节阻滞(SGB)模型和心脏不停跳体外循环（CPB）模型，检测CPB后大鼠海马caspase-3mRNA及蛋白的表达，探讨CPB是否对大鼠海马造成损伤，观察右侧SGB对CPB后大鼠海马caspase-3mRNA及蛋白表达的影响，探讨右侧SGB在CPB的脑损伤中是否有保护作用及其相关的作用机制。 方法：选用40只SPF级SD大鼠随机分为S组，R组,C组和RC组4组，每组10只。S组为假手术组，仅插管不转体外循环。R组为单纯右侧星状神经节阻滞后插管不转体外循环组。C组为单纯体外循环组。RC组为右侧星状神经节阻滞加体外循环组。R组与RC组用0.25%布匹卡因0.2ml后入路行右侧星状神经节阻滞。C组和RC组转流1h结束体外循环停机后监测3h。R组、S组插管成功后监测观察4h终止实验。实验过程监测大鼠的生命体征、血气等。实验结束,快速分离大鼠海马组织，，部分-80℃保存备用于逆转录-聚合酶链反应（RT-PCR）检测caspase-3mRNA的表达。部分海马组织用中性甲醛固定后石蜡包埋切片,行免疫组化法检测海马神经元caspase-3蛋白的表达。部分用电镜观察海马神经元超微结构的改变。 结果：可以成功建立大鼠SGB和CPB模型。 C组和RC组大鼠海马组织的casepase-3mRNA与蛋白表达较S组升高，差异有统计学意义（P＜0.05），RC组海马组织的casepase-3mRNA表达与蛋白表达较C组的低，差异有统计学意义（P＜0.05）。S组和R组大鼠海马组织casepase-3mRNA与蛋白表达间的比较，差异无统计学意义（P＞0.05）。S组和R组的海马神经元结构基本正常，核仁明显，核膜清晰，染色质均匀，细胞核周围细胞器较多。C组和RC组的神经元超微结构明显损伤,核仁溶解消失，核皱缩，细胞核周围细胞器减少，出现明显的神经元凋亡现象。RC组神经细胞超微结构的损伤程度较C组的轻。 结论：大鼠SGB和CPB模型是用以研究SGB作用机制和心脏不停跳CPB损伤机制的理想动物模型。大鼠CPB后出现脑损伤，海马组织caspase-3mRNA和蛋白的表达明显增高，大鼠海马神经元超微结构明显破坏，出现凋亡现象。SGB可以抑制CPB后大鼠海马神经元caspase-3mRNA和蛋白的表达，减缓细胞凋亡进程，对CPB的脑损伤产生保护作用。
[Abstract]:Objective: to establish the right stellate ganglion block model and cardiopulmonary bypass (CPB) model in rats, to detect the expression of caspase-3mRNA and protein in hippocampus of rats after CPB, and to investigate whether CPB can damage the hippocampus of rats. To observe the effect of right SGB on the expression of caspase-3mRNA and protein in hippocampus of rats after CPB, and to explore the protective effect of right SGB on the brain injury of CPB and its related mechanism. Methods: forty SPF SD rats were randomly divided into group S (n = 10), group R (n = 10), group C (n = 10) and group RC (n = 4). Right stellate ganglion block and cardiopulmonary bypass group. Group C: cardiopulmonary bypass group. Group RC: right stellate ganglion block plus cardiopulmonary bypass group. Group R and RC group with 0.25% cloth. The right stellate ganglion block group (group C) and group RC (group RC) were treated with right stellate ganglion block (right stellate ganglion block) after 1 hour of cardiopulmonary bypass (CPB). After successful intubation was performed in group 3h.R (group S), the experiment was terminated for 4 hours. The vital signs, blood gas and so on were monitored during the experiment. At the end of the experiment, the hippocampal tissues of the rats were separated quickly, and some of them were stored at -80 鈩

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