骨形态发生蛋白-4、5、6、7在脑损伤并骨折大鼠中的表达及意义

发布时间：2018-05-30 02:23

本文选题：脑损伤 + 骨折　；参考：《广西医科大学》2016年博士论文

【摘要】：目的：本文旨在探讨BMP-4、5、6、7在脑损伤并骨折大鼠中的表达及意义,研究脑损伤促进骨折愈合的机制,为骨折、骨折延迟愈合以及骨折不愈合的临床治疗提供一定的理论依据。方法：将SD大鼠110只,测量体重后采用随机数字表随机分成3组,A组为空白对照组10只,B组为单纯胫骨骨折组50只,C组为脑损伤并骨折组50只,B组建立胫骨骨折模型,C组予以改良Feeney撞击器建立中度脑损伤并骨折模型。观察两组大鼠的一般状态,包括动物体重,精神状态,大小便情况,活动情况,进食量,饮水量等；脑组织损伤情况,实验后第1、2、3、5、7周取C组动物脑组织,行组织病理切片后行HE染色,在光学显微镜下观察受损脑组织不同时间段的变化情况；骨折的线表现：术后第1、2、3、5、7周对B组和C组拍摄正侧位X片了解骨折愈合情况,以BI一2000医学图像分析系统分析骨痂的灰度值；观察骨痂大小,从膝关节处离断手术侧胫骨,以游标卡尺测量骨痂的最大长度；骨痂的组织学观察,取出骨痂,组织切片HE染色观察骨折局部血肿、骨痂组织结构变化等。分别于1、2、3、5、7周测量大鼠血清和骨痂中BMP-4、5、6、7基因的mRNA水平以及蛋白含量。统计分析所得数据。结果：观察到脑损伤并骨折组大鼠在第2周开始活动逐渐增多,第4周左右基本恢复正常活动,早于单纯骨折组的第5周；脑损伤的组织学发现：早期脑组织损伤出血,继而出现空洞、坏死；骨折的X线检查发现脑损伤并骨折组2周折端有骨痂生长,3周可见大量骨痂生长,骨折线模糊；5周、7周骨折端大量骨痂生长,骨折线基本消失,骨痂出现时间早于单纯骨折组,在第2、3、5、7周骨痂灰度高于单纯骨折组(P0.05)；骨痂直径测量发现脑损伤并骨折组的骨痂直径明显大于单纯骨折组(P0.05)；骨痂的组织学观察到脑损伤并骨折组早期出现大量新生血管以及骨小梁,骨痂形成以及骨痂塑形早于单纯骨折组；两组大鼠的血清BMP-4、6、7基因的mRNA均呈高水平表达(P0.05),随时间变化,而血清BMP-5基因的mRNA则未见异常表达,且脑损伤并骨折组血清及骨痂中的BMP-4、6、7蛋白含量mRNA表达水平高于单纯骨折组(P0.05)；两组大鼠的血清BMP-4、6、7蛋白含量高(P0.05),随时间变化,而血清BMP-5蛋白含量未见异常,且脑损伤并骨折组血清及骨痂中的BMP-4、6、7蛋白含量高于单纯骨折组(P0.05)。结论：1：在中度脑损伤的大鼠动物模型中骨痂形成时间早,数量多,骨折的愈合早,说明脑损伤可能会通过骨形成蛋白调控骨痂的形成,进而加速骨折愈合。2：在中度脑损伤的大鼠动物模型中血清和骨痂的骨形态发生蛋白mRNA和蛋白的表达水平存在差异,其中BMP-4、6、7的mRNA和蛋白表达水平较高,可能促进骨折的愈合,而BMP-5的mRNA和蛋白表达水平较低,可能对进骨折的愈合影响不大。3：动物模型中血清和骨痂的骨形态发生蛋白的mRNA的表达与蛋白表达相一致,说明多种骨形态发生蛋白在基因的转录和翻译过程中的调控机制可能相似。
[Abstract]:Objective : To investigate the expression and significance of BMP - 4 , 5 , 6 and 7 in brain injury and fracture rats .
The brain tissue was injured by HE staining at the 1st , 2nd , 3rd , 5th and 7th weeks after the experiment , and the changes of the injured brain tissue were observed under the optical microscope .
The line manifestations of fracture were as follows : 1 , 2 , 3 , 5 , 7 weeks after operation , positive side X - ray films were taken to understand fracture healing in group B and group C , and the gray value of callus was analyzed by BI - 2000 medical image analysis ;
The size of callus was observed , the tibia was separated from the knee joint and the maximum length of callus was measured with vernier caliper ;
The expression of BMP - 4 , 5 , 6 , 7 mRNA and protein content in serum and callus of rats were measured at 1 , 2 , 3 , 5 and 7 weeks . The results showed that the activity of BMP - 4 , 5 , 6 , 7 genes increased gradually in the second week after injury of brain injury and fracture group .
Histological findings of brain injury revealed that early brain tissue damaged hemorrhage , followed by cavitation and necrosis .
X - ray examination revealed that there was callus growth in 2 weeks of fracture group , and a large number of callus growth was seen in 3 weeks , and the fracture line was blurred ;
After 5 weeks , 7 weeks fracture end mass callus growth , fracture line basically disappeared , callus appeared earlier than simple fracture group , in the 2nd , 3rd , 5th , 7th week the callus gray level was higher than simple fracture group ( P0.05 ) ;
The diameter of callus was found to be significantly larger than that of simple fracture group ( P0.05 ) .
The histological observation of callus showed that there appeared a large number of new blood vessels in the early stage of the brain injury and early fracture group , the formation of callus and the formation of callus were earlier than that of simple fracture group .
The levels of BMP - 4 , 6 , 7 mRNA in serum of both groups were higher than those in simple fracture group ( P0.05 ) .
The bone morphogenetic protein ( BMP - 4 , 6 , 7 ) content of bone morphogenetic protein ( BMP - 4 , 6 , 7 ) was higher than that in simple fracture group ( P0.05 ) . Conclusion : 1 : The expression level of BMP - 4 , 6 , 7 in the rat model of moderate brain injury is higher than that in simple fracture group ( P0.05 ) .
【学位授予单位】：广西医科大学
【学位级别】：博士
【学位授予年份】：2016
【分类号】：R651.15;R683

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