创伤性颅脑伤后小鼠皮层和海马Wnt3a和β-Catenin的表达变化

发布时间：2018-05-30 03:24

本文选题：创伤性颅脑伤 + Wnta　；参考：《国际神经病学神经外科学杂志》2017年02期

【摘要】：目的探讨创伤性脑损伤后小鼠皮层和海马Wnt3a和β-Catenin的表达及其意义。方法在小鼠创伤性脑损伤模型基础上,采用免疫印迹法和免疫组织化学法检测创伤性脑损伤后Wnt3a和β-Catenin的蛋白变化。结果 Wnt3a和β-Catenin在对照组的脑组织中表达较低。创伤性脑损伤后1 h,皮层和海马区β-Catenin和Wnt3 a的含量开始增加。与对照组相比,伤后6h和第7天,β-Catenin和Wnt3a蛋白表达显著增加(P0.05)。免疫荧光染色结果显示,脑损伤24 h后,β-Catenin和Wnt3a在小鼠皮层和海马表达量均增加。结论小鼠创伤性脑损伤后早期和恢复期,Wnt3a和β-Catenin的蛋白水平在皮层和海马显著增高,这提示Wnt3a和β-Catenin可能在创伤性脑损伤的病理生理机制中发挥着重要的作用。
[Abstract]:Objective to investigate the expression and significance of Wnt3a and beta -Catenin in the cortex and hippocampus of mice after traumatic brain injury. Methods based on the model of traumatic brain injury in mice, the changes of Wnt3a and beta -Catenin were detected by immunoblotting and immunohistochemistry. The Wnt3a and beta -Catenin in the brain tissue of the control group were in the control group. 1 h after traumatic brain injury, the content of beta -Catenin and Wnt3 a in the cortex and hippocampus began to increase. Compared with the control group, the expression of beta -Catenin and Wnt3a protein increased significantly on 6h and seventh days after injury (P0.05). The results of immunofluorescence staining showed that the expression of beta -Catenin and Wnt3a increased in the cortex and hippocampus of mice after brain damage 24 h. Conclusion in the early and recovery stages of traumatic brain injury in mice, the protein levels of Wnt3a and beta -Catenin are significantly increased in the cortex and hippocampus, which suggests that Wnt3a and beta -Catenin may play an important role in the pathophysiological mechanism of traumatic brain injury.
【作者单位】：第四军医大学西京医院神经外科;兰州军区兰州总医院心脏内科;兰州军区兰州总医院神经外科;
【基金】：国家自然科学青年科学基金项目81401020
【分类号】：R651.15

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