病毒转染骨髓基质干细胞在不同移植点对颅脑损伤的保护

发布时间：2018-05-31 13:38

本文选题：骨髓 + 干细胞移植　；参考：《中国组织工程研究》2016年36期

【摘要】：背景:骨髓基质干细胞可分化为神经细胞,能促进神经组织修复,但具体机制还未完全阐明。目的:探讨β神经生长因子重组腺病毒转染骨髓基质干细胞移植对颅脑损伤的保护作用机制。方法:(1)利用β神经生长因子重组腺病毒载体系统转染体外培养的大鼠骨髓基质干细胞,然后用β-巯5基乙醇对骨髓基质干细胞进行定向诱导分化;(2)选取210只SD大鼠,随机分为组:诱导转染骨髓基质干细胞组、诱导未转染骨髓基质干细胞组、诱导剂+培养基组、颅脑损伤组、假手术组,每组42只。制备颅脑损伤模型,按照不同时间点(12,24,36,48,72 h)进行相应的移植物治疗,分别于移植当天,移植1,2,3,4周采用NSS评分法进行神经功能评分;(3)选取75只SD大鼠,同上随机分为5组,每组15只。颅脑损伤模型制备24 h后进行相应的移植物治疗。移植当天,移植1,2,3,4周对各组动物进行NSS评分。移植当天,移植2,4周,分别处死各组5只动物,获得脑组织标本,检测超氧化物歧化酶、丙二醛水平。结果与结论:(1)在48 h内移植,移植后1,2周诱导转染骨髓基质干细胞组的NSS评分均显著低于诱导未转染骨髓基质干细胞移植组和颅脑损伤组(P0.05);各移植时间点,移植后3,4周,诱导转染骨髓基质干细胞移植组的NSS评分均显著低于诱导未转染骨髓基质干细胞移植组和颅脑损伤组(P0.05);(2)在大鼠损伤后24 h移植,移植第1周诱导转染骨髓基质干细胞组NSS评分显著低于颅脑损伤组(P0.05),移植第2,3,4周诱导转染骨髓基质干细胞移植组与诱导未转染骨髓基质干细胞移植组NSS评分均显著低于颅脑损伤组(P0.05);(3)移植后2周,诱导转染骨髓基质干细胞移植组的超氧化物歧化酶水平均显著高于诱导剂+培养基移植组和颅脑损伤组(P0.05),丙二醛水平显著低于上述两组(P0.05);(4)结果表明,β神经生长因子重组腺病毒转染骨髓基质干细胞在不同时间点移植治疗颅脑损伤大鼠,可以发挥出一定的神经保护作用。
[Abstract]:Background: bone marrow stromal cells can differentiate into nerve cells and promote nerve tissue repair, but the mechanism has not been fully elucidated. Aim: to investigate the protective mechanism of bone marrow stromal cells (BMSCs) transfected with recombinant adenovirus of 尾 nerve growth factor (NGF) on craniocerebral injury. Methods bone marrow stromal stem cells (BMSCs) were transfected by 尾 -NGF recombinant adenovirus vector system and then differentiated into BMSCs by 尾 -mercaptoethanol. 210 SD rats were selected. They were randomly divided into three groups: induced transfection of bone marrow stromal cells group, induced untransfected bone marrow stromal cells group, induction medium group, craniocerebral injury group, sham-operation group, 42 rats in each group. The model of craniocerebral injury was established and the corresponding graft therapy was carried out according to the different time points of 1224466 (4872 h). On the day of transplantation, 75 Sprague-Dawley (SD) rats were randomly divided into 5 groups. The nerve function scores were evaluated by NSS scoring method at 1, 2, 3 and 4 weeks after transplantation, and 75 SD rats were randomly divided into 5 groups, and 75 SD rats were randomly divided into 5 groups. There were 15 rats in each group. The model of craniocerebral injury was made 24 hours after the corresponding graft therapy. On the day of transplantation, the NSS score of each group was evaluated 4 weeks after transplantation. On the day of transplantation, 4 weeks after transplantation, 5 animals in each group were killed, brain tissue samples were obtained and superoxide dismutase (SOD) and malondialdehyde (MDA) levels were detected. Results and conclusion within 48 hours of transplantation, the NSS scores of bone marrow stromal cells transfected with bone marrow stromal cells at 1 and 2 weeks after transplantation were significantly lower than those in untransfected bone marrow stromal cells transplantation group and craniocerebral injury group, and at each transplantation time point, 3 weeks after transplantation, 4 weeks after transplantation, the score of NSS was significantly lower than that in untransfected bone marrow stromal cell transplantation group and brain injury group. The NSS score of induced transfection group was significantly lower than that of untransfected BMSCs transplantation group and craniocerebral injury group (P 0.05). In the first week of transplantation, the NSS score of the BMSCs group was significantly lower than that of the brain injury group (P 0.05). The NSS score of the BMSCs transplantation group and the induced BMSCs transplantation group was significantly lower than that of the brain injury group. It was lower than that in the craniocerebral injury group (P 0.05) 2 weeks after transplantation. The levels of superoxide dismutase (SOD) in the group of induced transfection of bone marrow stromal cells were significantly higher than those in the group of inductive medium transplantation and the group of craniocerebral injury, and the level of malondialdehyde (MDA) was significantly lower than that in the two groups mentioned above. Bone marrow stromal cells transfected with recombinant adenovirus were transplanted at different time points to treat rats with craniocerebral injury. Can play a certain neuroprotective role.
【作者单位】：河南大学淮河医院神经外科;
【分类号】：R651.15

【相似文献】