全身低能量光疗对重度烧伤大鼠早期全身炎症反应的影响

发布时间：2018-06-21 11:26

本文选题：光疗法 + 烧伤　；参考：《第二军医大学学报》2017年08期

【摘要】：目的探讨全身低能量光疗(LLLT)对重度烧伤大鼠全身炎症反应的影响。方法将50只SD大鼠随机分为空白对照组、单纯烧伤组、烧伤后单次短时光照组、烧伤后单次长时光照组和烧伤后多次短时光照组,每组10只。单次短时光照组、烧伤后单次长时光照组、烧伤后多次短时光照组大鼠在烧伤后均给予低强度LED光(640nm)照射全身,分别照射5min/d、15min/d、5min×3次/d。各组大鼠分别于造模前1d,造模后即刻,造模后第1、3、7、14天取尾静脉血检测血清肿瘤坏死因子α(TNF-α)、白细胞介素(IL)-1β、IL-6的含量,计数静脉血白细胞;并于造模后第3、7、14天测量创面面积,计算创面愈合率。结果与空白对照组相比,造模后第1天时单纯烧伤组及烧伤后单次短时光照组大鼠血清TNF-α含量升高(P0.05),而烧伤后单次长时光照组及烧伤后多次短时光照组分别于造模后第1、7天时升高(P0.05);各组大鼠血清IL-1β含量均在造模后第1天时下降(P0.05),后逐渐恢复,但恢复速度不一;单纯烧伤组及烧伤后多次短时光照组大鼠血清IL-6含量均在造模后第1天时升高(P0.05),后下降,但单纯烧伤组下降幅度大于烧伤后多次短时光照组,而烧伤后单次短时光照组于造模后第3天升高后下降,烧伤后单次长时光照组呈持续升高态势(P0.05)。单纯烧伤组及烧伤后单次长时光照组大鼠静脉血白细胞计数均于造模后第1天时升高并维持(P0.05),其余各组白细胞计数无明显改变。烧伤后单次短时光照组、烧伤后单次长时光照组和烧伤后多次短时光照组大鼠创面愈合率均高于单纯烧伤组(P0.05)。结论全身LLLT可改善严重烧伤大鼠血清炎症因子TNF-α、IL-1β、IL-6的含量及静脉血白细胞计数,促进创面愈合,且不同照射模式所产生的效果不一。
[Abstract]:Objective to investigate the effect of systemic low energy phototherapy (LLLTT) on systemic inflammation in rats with severe burn. Methods Fifty Sprague-Dawley rats were randomly divided into blank control group, simple burn group, single light group after burn, single long time illumination group after burn and multiple short time light group after burn, with 10 rats in each group. The rats in the single short time illumination group, the single long time light group after burn and the burn multiple time short time illumination group were exposed to low intensity LED 640nm after burn. The rats were irradiated 5 min / d ~ 15 min / d ~ (-1) 5 min 脳 3 times / d ~ (-1) 路d ~ (-1) ~ (-1) 路min ~ (-1) ~ (-1) 路d ~ (-1) 路d ~ (-1) respectively. The blood samples of tail vein were collected at 1 day before model making and immediately after model making, and the contents of TNF- 伪 TNF- 伪 and IL-1- 尾 -IL-6 in serum were measured at 714 days after modeling, and the area of wound was measured on the 3rd day of 714 days after model making, and the contents of TNF- 伪 and IL-1- 尾 -IL-6 in serum were counted, and the wound area was measured at 714 days after modeling. The wound healing rate was calculated. Results compared with the blank control group, The content of TNF- 伪 in the serum of the rats in the burn group and the light group after burn was increased on the 1st day after the model, while the content of TNF- 伪 was increased in the single long time light group and the multiple short time light group on the 7th day after burn, and the level of serum TNF- 伪 in each group was higher than that in the control group on the 7th day after the model making, and the content of TNF- 伪 in the serum of the rats was increased in each group. The contents of IL-1 尾 in serum of rats decreased on the 1st day after the establishment of the model and gradually recovered. But the recovery rate was not the same, the serum IL-6 content in the burn group and the light group increased on the 1st day after the model, then decreased, but the amplitude of the decrease in the simple burn group was greater than that in the multiple light group after burn. However, the single short time illumination group increased at the 3rd day after burn, and then decreased, while the single long time light group showed a sustained increase trend of P0. 05% (P 0. 05%). The white blood cell count in vein blood of rats in burn group and single long time light group increased on the first day after burn injury and maintained P0.05A, but the other groups had no obvious change in white blood cell count. The healing rate of burn wounds in the group of single short time illumination after burn, the group of single long time light after burn and the group of multiple times light after burn were all higher than that of the group of simple burn (P 0.05). Conclusion systemic LLLT can improve the content of serum inflammatory factor TNF- 伪, IL-1 尾 and IL-6, and promote wound healing in rats with severe burn, and the effects of different irradiation modes are different.
【作者单位】：第二军医大学长海医院烧伤科;
【基金】：国家自然科学基金(81401596) Supported by National Natural Science Foundation of China(81401596) 上海市自然科学基金(13ZR1408800) Natural Science Foundation of Shanghai(13ZR1408800) 第二军医大学长海医院“1255”学科建设计划(CH125540600) the“1255”Discipline Construction Project of Changhai Hospital of Second Military Medical University(CH125540600)
【分类号】：R644

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