肾上腺素β2受体对失血性休克大鼠肝脏和骨骼肌PGC-1表达的影响

发布时间：2018-06-24 17:28

本文选题：失血性休克 + 肾上腺素　；参考：《大连医科大学》2013年硕士论文

【摘要】：目的：本研究旨在探讨肾上腺素β2受体调控对失血性休克大鼠肝脏和骨骼肌PGC-1表达及预后的影响。方法： 102只大鼠建立失血性休克—复苏模型（放血量按22.5ml/kg体重计算，放血时间在10分钟内完成，维持30分钟)。随机分为对照组（sham）、休克组（SR）、干预组（PR）：对照组不进行任何操作；休克组建立失血性休克模型30分钟后进行复苏；干预组建立失血性休克模型后立即静脉给予ICI118.551，，30分钟后进行复苏。观察并记录各组大鼠的血流动力学变化：平均动脉压（MAP）和心率（HR）；记录大鼠生存时间并计算72小时生存率；使用定量PCR法和Western blotting法分别检测各组2小时、4小时、8小时和24小时肝脏和骨骼肌组织PGC-1mRNA和蛋白的表达变化情况。结果： 1、生存率：三组大鼠72小时生存率分别为100％、50%、80%。平均生存时间分别为72.00±0.00h、48.00±8.25h、62.40±3.40h。休克组和干预组间比较有统计学差异（p0.05）。 2、血流动力学：休克组大鼠MAP在各时间点上的变化与干预组比较无统计学差异（p＞0.05）。休克组大鼠HR在各时间点上的变化与干预组比较没有统计学差异（p＞0.05）。 3、肝脏和骨骼肌组织PGC-1mRNA表达变化：干预组在复苏后2小时PGC-1mRNA表达显著低于休克组，与休克组比较有统计学差异（p0.05）。其它时间点休克组和干预组PGC-1mRNA表达比较无统计学差异（p0.05）。 4、肝脏和骨骼肌组织PGC-1蛋白表达变化：干预组在复苏后2小时PGC-1表达显著低于休克组，与休克组比较有统计学差异（p0.05）。其它时间点休克组和干预组PGC-1蛋白表达比较无统计学差异（p0.05）。结论： 1、阻断肾上腺素β2受体可以提高失血性休克大鼠72小时生存率。 2、阻断肾上腺素β2受体可短暂下调失血性休克大鼠肝组织和骨骼肌组织PGC-1基因和蛋白的表达。 3、阻断肾上腺素β2受体可能通过下调PGC-1的表达，减少失血性休克大鼠的能量消耗，进而提高生存率。
[Abstract]:Aim: to investigate the effects of adrenaline 尾 2 receptor regulation on the expression and prognosis of PGC-1 in liver and skeletal muscle of hemorrhagic shock rats. Methods: the model of hemorrhagic shock and resuscitation was established in 102 rats (the blood volume was calculated according to 22.5ml/kg body weight, the bleeding time was completed in 10 minutes and maintained for 30 minutes). (sham), shock group (SR) was randomly divided into control group (SR), intervention group (PR): the control group did not perform any operation, the shock group established hemorrhagic shock model 30 minutes after resuscitation, and the intervention group was resuscitated immediately after the establishment of hemorrhagic shock model by intravenous administration of ICI 118.551h for 30 minutes. The mean arterial pressure (map) and heart rate (HR), survival time and 72 hour survival rate were observed and recorded. The expression of PGC-1 mRNA and protein in liver and skeletal muscle were detected by quantitative PCR and Western blotting. Results: 1, survival rate: the 72-hour survival rate of the three groups was 100 and 50 / 80, respectively. The mean survival time was 72.00 卤0.00h, 48.00 卤8.25h, 62.40 卤3.40h, respectively. There was significant difference between shock group and intervention group (p0.05). 2Hemodynamics: there was no significant difference in map between shock group and intervention group at each time point (p > 0.05). The changes of HR in shock group were not significantly different from those in intervention group (p > 0.05). 3. The expression of PGC-1 mRNA in liver and skeletal muscle was significantly lower in intervention group than that in shock group at 2 hours after resuscitation. Compared with shock group, there was statistical difference (p0.05). There was no significant difference in PGC-1mRNA expression between shock group and intervention group at other time points (p0.05). 4. The expression of PGC-1 protein in liver and skeletal muscle was significantly lower than that in shock group at 2 hours after resuscitation. Compared with shock group, there was statistical difference (p0.05). There was no significant difference in PGC-1 protein expression between shock group and intervention group at other time points (p0.05). Conclusion: 1. Blocking adrenaline 尾 2 receptor can improve 72 hour survival rate of hemorrhagic shock rats. 2. Blocking adrenaline 尾 2 receptor can temporarily downregulate liver tissue and skeletal muscle of hemorrhagic shock rats. The expression of PGC-1 gene and protein. 3. Blocking adrenaline 尾 2 receptor may down-regulate the expression of PGC-1. The energy consumption of hemorrhagic shock rats was reduced and the survival rate was improved.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R459.7

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