封闭负压引流技术促进慢性创面愈合机制的研究

发布时间：2018-07-11 21:58

本文选题：封闭负压引流 + 慢性创面　；参考：《苏州大学》2013年硕士论文

【摘要】：目的研究慢性创面中表皮生长因子(epidermal growth factor，EGF)、血小板衍化生长因子(platelet derived growth factor，，PDGF)、血管内皮细胞生长因子(vascular endothelialgrowth factor，VEGF)等生长因子及其受体表达的变化，探讨封闭负压引流技术(vacuum sealing drainage，VSD)促进慢性创面愈合的机制。方法选取我院2011年1月-2012年12月慢性创面患者40例，随机分为治疗组和对照组，每组20人。治疗组创面用封闭负压引流治疗，对照组创面用传统换药治疗。两组均在治疗前及治疗后1周取创面组织5mm×5mm。采用光镜、免疫组化等方法，观察创面愈合过程中EGF、PDGF及VEGF等生长因子及其受体的表达的变化。结果治疗组病人创面肉芽组织比较新鲜，血供丰富，而对照组病人创面则无明显变化。光镜见治疗组治疗后1周创面组织切片中蓝染部分明显比对照组明显增加。免疫组化见治疗组创面组织中EGF、PDGF及VEGF及其受体表达明显比对照组增多，且两者差异均有统计学意义(P0.05)。结论封闭负压引流能改善创面血液循环，促进创面肉芽组织生长，增加创面组织中EGF、PDGF、VEGF等生长因子及其受体的表达，从而加速创面愈合。
[Abstract]:Objective to study the expression of epidermal growth factor (epidermal growth), platelet-derived growth factor (platelet derived growth) and vascular endothelial growth factor (vascular endothelialgrowth) in chronic wounds. Objective: to investigate the mechanism of (vacuum sealing drainage (VSD) in promoting the healing of chronic wounds. Methods from January 2011 to December 2012, 40 patients with chronic wounds in our hospital were randomly divided into treatment group and control group with 20 patients in each group. The wounds in the treatment group were treated with closed negative pressure drainage, while the wounds in the control group were treated with traditional dressing change. 5mm 脳 5 mm of wound tissue were taken before and 1 week after treatment in both groups. The expression of EGF- PDGF and VEGF and their receptors during wound healing were observed by light microscopy and immunohistochemistry. Results the granulation tissue was fresh and blood supply was abundant in the treatment group, but there was no obvious change in the control group. The blue staining in the tissue sections of the wounds in the treatment group was significantly higher than that in the control group 1 week after treatment. Immunohistochemistry showed that the expression of EGF- PDGF, VEGF and its receptor in the wound tissue of the treatment group was significantly higher than that of the control group, and the difference between the two groups was statistically significant (P0.05). Conclusion blocking negative pressure drainage can improve the blood circulation of wound, promote the growth of granulation tissue, increase the expression of VEGF and its receptor in wound tissue, and accelerate wound healing.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R641

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