严重烧伤大鼠早期山莨菪碱治疗对肠源性真菌感染防治作用的研究

发布时间：2018-07-13 21:45

【摘要】：目的研究早期应用山莨菪碱在大鼠烧伤后肠源性真菌感染的作用,从而为烧伤患者临床治疗提供新方法的依据。方法选取健康大鼠72只,随机分成3组,A组健康鼠真菌液灌胃,B组烧伤鼠真菌液灌胃,C组烧伤鼠真菌液灌胃5分钟内山莨菪碱(3mg/kg)腹腔注射,设计严重烧伤大鼠动物模型,选用标准白色念珠菌菌株制备一定浓度,给烧伤大鼠灌肠,分不同时段(6h,12h,24h,48h)处死,取各组大鼠内脏组织,肺部组织进行MPO ELISA半定量测定,小肠粘膜进行HSP-70ELISA半定量测定,并应用实时荧光定量PCR技术对肠道粘膜、肺、肝、肾脏真菌含量进行检测,同时抽血测定血清丙氨酸转移酶(ALT)、天门冬氨酸转移酶(AST)、肌酐(CRE)尿素氮(BUN),分析不同时相点的组织真菌含量变化,脏器功能指标变化,以探讨山莨菪碱对严重烧伤大鼠烧伤后肠源性真菌感染的作用。结果肺组织MPO测定结果显示B组(烧伤真菌液灌胃)、C组(烧伤鼠真菌液灌胃5分钟内山莨菪碱腹腔注射)各时相点MPO含量显著高于A组(健康鼠真菌液灌胃)；B组MPO含量显著高于C组。小肠粘膜HSP70测定结果显示各时相点B组、C组含量明显高于A组；而且C组含量高于B组。丙氨酸转移酶(ALT)、天门冬氨酸转移酶(AST)B组显著高于C组高于A组。血清肌酐(CRE)尿素氮(BUN)测定结果显示B组、C组在24、48明显升高,与A组比较有显著差异,C组与B组比较C组明显低于B组；各脏器荧光定量PCR检测真菌显示均有不同程度侵袭,侵袭程度A组最轻、C组次之,B组最重。结论严重烧伤大鼠肠源性真菌感染后真菌侵袭范围广,随时间延长受感染脏器损伤程度加重；早期使用山莨菪碱可减轻烧伤大鼠的肠源性真菌感染程度,减轻其它脏器的感染程度,保护损伤脏器；实时荧光定量PCR法可作为一种快速检测方法应用于真菌感染的诊断。
[Abstract]:Objective to study the effect of early application of anisodamine on intestinal fungal infection after burn in rats, so as to provide a new method for clinical treatment of burn patients. Methods Seventy-two healthy rats were randomly divided into 3 groups: group A, normal rats were treated with fungous fluid in group B, group C were given intraperitoneal injection of anisodamine (3mg/kg) within 5 minutes, and the animal model of severe burn was designed. Standard Candida albicans strain was used to prepare a certain concentration of Candida albicans. The rats were enema and killed in different time periods (6 h, 12 h, 24 h, 48 h). The visceral tissue and lung tissue of each group were measured by MPO Elisa, and the intestinal mucosa was detected by HSP-70 Elisa. The fungal contents of intestinal mucosa, lung, liver and kidney were detected by real-time fluorescent quantitative PCR. Serum alanine transferase (alt), aspartate transferase (AST), creatinine (CRE) urea nitrogen (bun) were measured. To investigate the effect of anisodamine on intestinal fungal infection after severe burn in rats. Results MPO of lung tissue in group B was significantly higher than that in group A (intraperitoneal injection of anisodamine within 5 minutes). MPO content in group B was significantly higher than that in group C. The results of HSP70 assay showed that the content of HSP70 in group B was significantly higher than that in group A, and that in group C was higher than that in group B. Alanine transferase (alt) and aspartate transferase (AST) were significantly higher in group B than in group C than in group A. The serum creatinine (CRE) urea nitrogen (bun) level in group B was significantly higher than that in group A, which was significantly lower in group C than in group B, and in group C was significantly lower than that in group B. The degree of invasion was the lightest in group A and the lowest in group C, followed by group B. Conclusion severe burn rats have a wide range of fungal invasion after enterogenous fungi infection, and the degree of organ injury is aggravated with time, and early use of anisodamine can reduce the degree of intestinal fungal infection in burn rats. The real-time fluorescence quantitative PCR can be used as a rapid detection method for the diagnosis of fungal infection.
【学位授予单位】：内蒙古大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R644;R965

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