辛伐他汀对大鼠烟雾吸入性肺损伤中炎症反应的抑制作用

发布时间：2018-07-30 08:16

【摘要】：目的研究辛伐他汀对大鼠烟雾吸入性肺损伤中炎症反应的抑制作用。方法将18只SD大鼠(雌雄不限)随机分为正常组、盐水组、辛伐他汀组,建立烟雾吸入性肺损伤模型,模型建立后30 min及12 h,辛伐他汀组给予辛伐他汀溶液50 mg/kg灌胃,盐水组大鼠给予等量生理盐水灌胃,正常组大鼠正常饲养。于致伤后24 h取右上肺肺组织行病理检查并行病理学评分。经腹主动脉取全血离心留取上层血清,并行左侧支气管肺泡灌洗留取肺泡灌洗液(BALF),采用ELISA法检测血清及BALF中白介素(IL)-6、肿瘤坏死因子(TNF)-α的含量。取右下肺部分肺组织以Western blot法检测蛋白提取物中IL-6、TNF-α及胞质中NF-κB p65的蛋白表达情况。结果光镜下观察正常组肺泡结构正常,盐水组肺泡间隔水肿,肺泡腔内可见大量的中性粒细胞浸润,而与盐水组相比,辛伐他汀组上述症状减轻且肺组织病理学评分降低(P0.05)。盐水组、辛伐他汀组血清和BALF中IL-6、TNF-α的含量以及肺组织蛋白提取物中IL-6、TNF-α和NF-κB的表达均较正常组明显升高(P0.05)。而与盐水组相比,辛伐他汀组大鼠血清和BALF中IL-6、TNF-α的含量以及肺组织蛋白提取物中IL-6、TNF-α及NF-κB p65的表达均降低(P0.05)。结论辛伐他汀通过抑制炎症介质的产生及炎症细胞的浸润,减轻吸入性肺损伤的炎症反应,对肺组织起到了一定的保护作用。
[Abstract]:Objective to study the inhibitory effect of simvastatin on inflammatory response in rats with smoke inhalation lung injury. Methods 18 SD rats were randomly divided into normal group, saline group and simvastatin group. Smoke inhalation lung injury model was established. 30 min and 12 h after establishment, simvastatin group was given simvastatin solution for 50 mg/kg. Rats in the saline group were fed with the same amount of normal saline, while the rats in the normal group were fed normally. The right upper lung tissue was taken 24 hours after injury for pathological examination and pathological score. The serum of whole blood was collected from abdominal aorta by centrifugation, and the left bronchoalveolar lavage fluid (BALF),) was used to detect the levels of interleukin (IL) 6 and tumor necrosis factor (TNF)-伪 in serum and BALF by ELISA method. The expression of IL-6 TNF- 伪 in protein extract and NF- 魏 B p65 in cytoplasm were detected by Western blot method. Results the alveolar structure of normal group was normal, the alveolar septum edema and neutrophil infiltration could be seen in the alveolar cavity of saline group. Compared with saline group, simvastatin group had less symptoms and lower lung histopathological score (P0.05). The levels of IL-6 TNF- 伪 in serum and BALF and the expression of IL-6 TNF- 伪 and NF- 魏 B in protein extracts of lung tissue in saline group and simvastatin group were significantly higher than those in normal group (P0.05). Compared with the saline group, the levels of IL-6TNF- 伪 in serum and BALF and the expression of IL-6TNF- 伪 and NF- 魏 B p65 in the lung tissue protein extract were decreased in simvastatin group (P0.05). Conclusion Simvastatin can attenuate the inflammatory response of inhaled lung injury by inhibiting the production of inflammatory mediators and the infiltration of inflammatory cells and plays a protective role in lung tissue.
【作者单位】：郑州大学第一附属医院烧伤与修复重建外科;中国人民解放军火箭军总医院礼士路门诊部;
【基金】：国家自然科学基金(编号:U1604188) 2015年河南省重点科技攻关项目(编号:152102410065)
【分类号】：R644

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