丹参注射液对神经干细胞移植治疗颅脑损伤的影响

发布时间：2018-08-06 11:01

【摘要】：背景:丹参提取液可显著提高神经干细胞的增殖活性,改善神经干细胞移植的存活。目的:探讨丹参注射液对神经干细胞蛛网膜下腔移植治疗大鼠颅脑损伤的影响。方法:选取87只成年SD大鼠制成重型液压颅脑损伤模型,将建模成功的80只随机分4组干预,脑损伤组蛛网膜下腔注射DMEM培养液,1次/d;丹参注射液组腹腔注射丹参注射液,1次/d;干细胞组蛛网膜下腔注射CM-Dil标记的神经干细胞悬液,1次/d;联合组蛛网膜下腔注射CM-Dil标记神经干细胞细胞悬液的同时腹腔注射丹参注射液,1次/d,各组连续干预3 d。干预后3 d及1,2,3,4周行神经学缺损评分;干预21-28 d进行为期7 d的Morris水迷宫实验;干预后4周进行脑组织病理组织学观察、神经干细胞存活与分布观察、RT-PCR及Western blot检测。结果与结论:①神经学缺损评分:联合组神经学缺损评分始终优于其余3组(P0.05);②Morris水迷宫实验:联合组干预后3-5 d的平均潜伏时间明显短于其他3组(P0.05或P0.01),穿越平台次数及在目标象限游泳距离与总距离百分比均高于其他3组(P0.05或P0.01);③脑组织病理组织学观察:脑损伤组可见脑损伤区组织空洞明显,丹参注射液组、干细胞组脑损伤区组织空洞较小,联合组脑损伤区组织空洞几乎消失;④神经干细胞存活与分布:联合组CM-Dil标记阳性细胞数多于干细胞组;⑤RT-PCR及Western blot检测:联合组脑组织突触素及生长相关蛋白43表达高于其他3组(P0.05),丹参注射液组、干细胞组高于脑损伤组(P0.05);⑥结果表明:丹参注射液联合神经干细胞蛛网膜下腔移植可能通过促进大鼠脑损伤区脑组织突触素及生长相关蛋白43的表达,改善重型颅脑损伤模型大鼠的神经功能。
[Abstract]:Background: Salvia miltiorrhiza extract can significantly improve the proliferation activity of neural stem cells and the survival of neural stem cell transplantation. Objective: to investigate the effect of Salvia miltiorrhiza injection on subarachnoid transplantation of neural stem cells in the treatment of craniocerebral injury in rats. Methods: 87 adult Sprague-Dawley rats were selected to make severe hydraulic craniocerebral injury model and 80 rats were randomly divided into 4 groups. Brain injury group subarachnoid injection of DMEM culture solution 1 / d; Salvia miltiorrhiza injection group intraperitoneal injection of salvia miltiorrhiza injection 1 / d; stem cell group subarachnoid injection of CM-Dil labeled neural stem cell suspension 1 / d; combined group subarachnoid cavity injection CM-Dil labeled neural stem cell suspension was injected intraperitoneally into Salvia miltiorrhiza injection for 3 days. The neurological defect score was evaluated 3 days after intervention and 3 weeks after intervention, the Morris water maze test was conducted for 7 days from 21 to 28 days, the histopathology of brain tissue, the survival and distribution of neural stem cells were observed by RT-PCR and Western blot detection at 4 weeks after intervention. Results and conclusion: the neurological defect score of the combined group was always better than that of the other three groups (P0.05). The average latency time of the combined group was significantly shorter than that of the other three groups (P0.05 or P0.01), and the mean latency time of the combined group was significantly shorter than that of the other three groups (P0.05 or P0.01). The frequency of platform and the percentage of swimming distance and total distance in the target quadrant were higher than those in the other three groups (P0.05 or P0.01). In the Salvia miltiorrhiza injection group, the cavities in the brain injury area of the stem cell group were small, and the cavities in the brain injury area in the combined group almost disappeared. The number of CM-Dil labeled positive cells in the combined group was more than that in the stem cell group. 5RT-PCR and Western blot: the expression of synaptophysin and growth-associated protein 43 in brain tissue of combined group was higher than that of other three groups (P0.05), and that of Salvia miltiorrhiza injection group was higher than that of other three groups. The results showed that salvia miltiorrhiza injection combined with neural stem cell subarachnoid transplantation might promote the expression of synaptophysin and growth-associated protein 43 in brain tissue of rats with brain injury. To improve the neurological function of rats with severe craniocerebral injury.
【作者单位】：天津市津南区咸水沽医院脑系科;天津市环湖医院神经外科五病区;
【基金】：天津市卫生行业重点攻关项目(2013KG134)~~
【分类号】：R651.15

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