依达拉奉对高胆红素血症脑损伤新生大鼠血清S100B蛋白水平的影响
发布时间：2018-08-12 11:01
【摘要】：目的:观察高胆红素血症(hyperbilirubinemia)脑损伤新生大鼠血清S100B蛋白水平及依达拉奉(edaravone,MCI-186)干预后其浓度的变化。探讨依达拉奉对高胆红素血症脑损伤的作用,为临床上依达拉奉防治胆红素脑病提供一定的实验依据。方法:将96只7日龄SD(Sprague-Dawley,SD)大鼠随机分为3组:正常对照组、高胆红素血症组、依达拉奉干预组,即A、B、C三组,每组32只大鼠。A、B、C组再依据处死时间点分为4个亚组:24h组、48h组、72h组和96h组,每组8只大鼠。采取腹腔注射的方法给B、C组大鼠按100mg/kg的剂量注入1次胆红素,用同样方法给A组大鼠按同样剂量注入1次生理盐水,造模后C组大鼠立即接受依达拉奉干预,使用同样方法注入2mg/kg/d,连续4天,最后在各不同处死时间点尽快将各组大鼠的血清及脑组织取出。用HE(Hematoxylin and eosin)染色方法观察大鼠脑组织的病理学改变,用Elisa法测血清中S100B蛋白的浓度,然后记录实验结果、计算相关数据并对其进行统计学分析,并得出结论。结果:(1)造模后,明显的神经行为异常均出现在B、C组大鼠,各不同时间点C组大鼠症状较B组的明显改善,而A组大鼠行为无明显异常。(2)造模24h后,B、C组大鼠脑组织出现水平不一的水肿、变性及炎性浸润等,随着时间延长其程度加重。各处死时间点C组大鼠脑组织改变程度较B组的减轻,A组大鼠脑组织结构基本正常。(3)B组大鼠血清S100B蛋白的浓度显著高于A、C两组,且和时间呈正相关性,差异存在统计学意义(P0.05);C组大鼠血清S100B蛋白的浓度显著低于B组,高于A组,差异存在统计学意义(P0.05);A组大鼠血清S100B蛋白水平较低,各处死时间点组内相比,差异没有统计学意义(P0.05)。结论:(1)高胆红素血症时,大鼠血清S100B蛋白水平明显升高,且和时间呈正相关性;经药物干预后,血清S100B蛋白水平明显降低。(2)高胆红素血症时,24h后大鼠脑组织出现水肿、变性及炎性浸润等改变,且随着时间延长,其程度有所加重;经依达拉奉干预后,不同时间点大鼠脑组织的水肿、变性及炎性浸润等可被不同程度减轻.(3)表明依达拉奉可减轻过量胆红素对中枢神经系统的损伤,起到保护神经元的作用。
[Abstract]:Aim: to observe the changes of serum S100B protein and the concentration of Edaravone MCI-186 in neonatal rats with hyperbilirubinemia (hyperbilirubinemia) brain injury. To investigate the effect of Edaravone on cerebral injury of hyperbilirubinemia and to provide experimental evidence for the prevention and treatment of bilirubin encephalopathy by Edaravone. Methods: Ninety-six 7-day-old SD rats were randomly divided into three groups: normal control group, hyperbilirubinemia group, Edaravone intervention group, Acarb Bon C group. The 32 rats in each group were divided into 4 subgroups according to the time point of death. They were divided into 4 subgroups: 24 h group, 48 h group, 72 h group and 96 h group, 8 rats in each group. Rats in group C were injected bilirubin once according to the dose of 100mg/kg, and rats in group A were injected with normal saline at the same dose by intraperitoneal injection. The rats in group C were treated with Edaravone immediately after the establishment of the model. The same method was used to inject 2 mg / kg / d for 4 consecutive days. Finally, the serum and brain tissues of each group were removed as soon as possible at different time points. The pathological changes of brain tissue in rats were observed by HE (Hematoxylin and eosin) staining. The concentration of S100B protein in serum was measured by Elisa method. The experimental results were recorded, the relevant data were calculated and statistically analyzed, and the conclusion was reached. Results: (1) after modeling, the obvious neurobehavioral abnormalities were found in rats of group B, and the symptoms of group C were significantly improved compared with those of group B at different time points. But there was no obvious abnormal behavior in group A. (2) after 24 hours of modeling, there were edema, degeneration and inflammatory infiltration in brain tissue of rats in group B and C, which increased with time. The changes of brain tissue in group C were normal compared with those in group B. (3) the concentration of serum S100B protein in group B was significantly higher than that in group A and C, and had a positive correlation with time. The difference was statistically significant (P0.05) the concentration of serum S100B protein in group C was significantly lower than that in group B and higher than that in group A (P0.05). The level of S100B protein in group A was lower than that in group A (P0.05). Conclusion: (1) at hyperbilirubinemia, the serum S100B protein level was significantly increased and positively correlated with the time, and the serum S100B protein level was significantly decreased after drug intervention. (2) there was edema in the brain tissue of rats with hyperbilirubinemia at 24 h after hyperbilirubinemia. The degree of degeneration and inflammatory infiltration was increased with time. After Edaravone intervention, edema of brain tissue was observed in rats at different time points. Denaturation and inflammatory infiltration can be alleviated in varying degrees. (3) Edaravone can reduce the damage of excessive bilirubin to the central nervous system and protect neurons.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R651.15
本文编号：2178850
[Abstract]:Aim: to observe the changes of serum S100B protein and the concentration of Edaravone MCI-186 in neonatal rats with hyperbilirubinemia (hyperbilirubinemia) brain injury. To investigate the effect of Edaravone on cerebral injury of hyperbilirubinemia and to provide experimental evidence for the prevention and treatment of bilirubin encephalopathy by Edaravone. Methods: Ninety-six 7-day-old SD rats were randomly divided into three groups: normal control group, hyperbilirubinemia group, Edaravone intervention group, Acarb Bon C group. The 32 rats in each group were divided into 4 subgroups according to the time point of death. They were divided into 4 subgroups: 24 h group, 48 h group, 72 h group and 96 h group, 8 rats in each group. Rats in group C were injected bilirubin once according to the dose of 100mg/kg, and rats in group A were injected with normal saline at the same dose by intraperitoneal injection. The rats in group C were treated with Edaravone immediately after the establishment of the model. The same method was used to inject 2 mg / kg / d for 4 consecutive days. Finally, the serum and brain tissues of each group were removed as soon as possible at different time points. The pathological changes of brain tissue in rats were observed by HE (Hematoxylin and eosin) staining. The concentration of S100B protein in serum was measured by Elisa method. The experimental results were recorded, the relevant data were calculated and statistically analyzed, and the conclusion was reached. Results: (1) after modeling, the obvious neurobehavioral abnormalities were found in rats of group B, and the symptoms of group C were significantly improved compared with those of group B at different time points. But there was no obvious abnormal behavior in group A. (2) after 24 hours of modeling, there were edema, degeneration and inflammatory infiltration in brain tissue of rats in group B and C, which increased with time. The changes of brain tissue in group C were normal compared with those in group B. (3) the concentration of serum S100B protein in group B was significantly higher than that in group A and C, and had a positive correlation with time. The difference was statistically significant (P0.05) the concentration of serum S100B protein in group C was significantly lower than that in group B and higher than that in group A (P0.05). The level of S100B protein in group A was lower than that in group A (P0.05). Conclusion: (1) at hyperbilirubinemia, the serum S100B protein level was significantly increased and positively correlated with the time, and the serum S100B protein level was significantly decreased after drug intervention. (2) there was edema in the brain tissue of rats with hyperbilirubinemia at 24 h after hyperbilirubinemia. The degree of degeneration and inflammatory infiltration was increased with time. After Edaravone intervention, edema of brain tissue was observed in rats at different time points. Denaturation and inflammatory infiltration can be alleviated in varying degrees. (3) Edaravone can reduce the damage of excessive bilirubin to the central nervous system and protect neurons.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R651.15
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