不同液体复苏对失血性休克大鼠电解质及凝血功能等指标的影响

发布时间：2018-08-20 14:30

【摘要】：目的:探讨不同液体复苏对失血性休克大鼠电解质及凝血功能等指标的影响。方法:将60只12周龄SD大鼠随机分为4组:NC组(空白对照组)仅对大鼠进行手术并监测但不进行放血;NF组(失血性休克组)对大鼠进行手术和监测并放血建立失血性休克模型但不予液体复苏;常规液体复苏组在大鼠休克模型建立后使用4%琥珀酰明胶及复方氯化钠注射液进行充分补液将血压恢复至正常水平(80mmHg);限制性液体复苏组使用4%琥珀酰明胶及7.5%高渗盐水对大鼠进行复苏仅将血压控制在60mmHg,每组15只大鼠中10只进行手术,如出现大鼠死亡或复苏效果不满意则由余下5只中大鼠进行替代,休克模型建立后经由间断放血和血液回输的方法维持休克血压60min,对不同组别SD大鼠选择性的进行液体复苏,按需要及不同方法复苏至要求血压(常规液体复苏组75-85mmHg,限制性液体复苏组55-65mmHg)后30min取大鼠外周血进行血中电解质及凝血功能的检测,处死大鼠。结果:与NC组相比,各组大鼠均出现较为明显的电解质紊乱及凝血功能异常,其中NF组即未复苏组最为严重,限制性液体复苏组各项指标明显优于非限制性液体复苏组,两组中各项指标的比较差异有统计学意义(P0.05)。结论:与非制性液体复苏比较,限制性液体复苏能够更好地改善失血性休克大鼠的凝血功能异常并纠正电解质紊乱,对于失血性休克大鼠的抗休克治疗优于非限制性液体复苏。
[Abstract]:Objective: to investigate the effects of fluid resuscitation on electrolyte and coagulation function in hemorrhagic shock rats. Methods: sixty 12-week-old SD rats were randomly divided into 4 groups: the control group (control group) and the control group. The rats were operated on and monitored only in the control group, but not in the NF group (hemorrhagic shock group). Hemorrhagic shock model but no fluid resuscitation; Normal fluid resuscitation group used 4% succinyl gelatin and compound sodium chloride injection to restore blood pressure to normal level (80mmHg) after the rat shock model was established, and restrictive fluid resuscitation group used 4% succinyl gelatin and 7.5% high level. The rats were resuscitated by osmotic saline and the blood pressure was controlled to 60 mm Hg.The operation was performed on 10 of 15 rats in each group. If the rats died or if the resuscitation effect was unsatisfactory, they were replaced by the remaining 5 rats. After the establishment of the shock model, the shock blood pressure was maintained by intermittent bleeding and blood transfusion for 60 min, and the fluid resuscitation was carried out selectively to different groups of SD rats. The rats were resuscitated to the required blood pressure (75-85mm Hg in conventional fluid resuscitation group, 55-65mmHg in restricted fluid resuscitation group) according to the need and different methods. The peripheral blood electrolyte and coagulation function of rats were measured by 30min. The rats were killed. Results: compared with NC group, the electrolyte disturbance and coagulation dysfunction were more obvious in all groups. NF group was the most serious group, and the restrictive fluid resuscitation group was superior to the non-restrictive fluid resuscitation group. The difference between the two groups was statistically significant (P0.05). Conclusion: compared with non-fluid resuscitation, restrictive fluid resuscitation can improve coagulation dysfunction and correct electrolyte disturbance in hemorrhagic shock rats, and antishock therapy is better than non-restrictive fluid resuscitation in hemorrhagic shock rats.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R459.7

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