自噬在急性心肌梗死后无复流中的表达
发布时间：2018-08-30 12:10
【摘要】：目的观察急性心肌梗死后无复流区心肌细胞自噬的表达,探讨心肌细胞自噬在大鼠实验性无复流中的作用。方法 SD大鼠随机分为实验组和对照组:均予结扎冠状动脉2 h,再灌注1 h,建立急性心肌梗死后再灌注无复流模型,对照组只穿线,不结扎。在再灌注末,从左心房注入6%硫磺素1 m L/kg使复流区着色,无复流区不着色;再于原位重新结扎冠状动脉,实验组从左心房注入Evan'S蓝,使结扎区外着蓝色,梗死区不着蓝色,对照组不予Evan's蓝。采用实时定量PCR法和Western blot法检测实验组无复流区、梗死区和正常区心肌组织自噬相关蛋白LC3、p62/SQSTM1表达;电镜下观察自噬体超微结构改变情况。结果 TTC和硫磺素染色结果显示,实验组无复流面积显著高于对照组(P0.05);透射电镜观察到,大鼠心肌细胞内自噬体体积增大,数量增多;PCR法和Western blot法检测实验组无复流区、梗死区及正常区心肌组织自噬相关蛋白LC3-Ⅰ、LC3-Ⅱ,p62/SQSTM1的表达,实验组无复流区自噬表达明显高于梗死区和正常区心肌组织(P0.05),但梗死区和远端区心肌组织自噬表达差异无统计学意义(P0.05)。结论实验组无复流区心肌组织局部自噬表达增强,其活性的改变可能与无复流的发展及程度相关。
[Abstract]:Objective to observe the expression of myocardial autophagy in non-reflow zone after acute myocardial infarction (AMI) and to investigate the role of myocardial autophagy in experimental non-reflow rats. Methods SD rats were randomly divided into experimental group and control group: coronary artery was ligated for 2 hours and reperfusion for 1 hour. At the end of reperfusion, the reflow area was stained by injecting 6% sulfur 1 m L/kg from the left atrium, and the no reflow area was not stained, and then the coronary artery was religated in situ, and the Evan'S blue was injected into the left atrium in the experimental group, so that the ligation area was blue outside the ligation area and the infarcted area was not blue. The control group was not treated with Evan's blue. Real time quantitative PCR and Western blot were used to detect the expression of autophagy associated protein (LC3,p62/SQSTM1) in myocardium without reflow, infarction and normal myocardium, and ultrastructural changes of autophagy were observed under electron microscope. Results the results of TTC and sulfur staining showed that the no reflow area in the experimental group was significantly higher than that in the control group (P0.05). Expression of autophagy associated protein LC3- 鈪，
本文编号：2213033
[Abstract]:Objective to observe the expression of myocardial autophagy in non-reflow zone after acute myocardial infarction (AMI) and to investigate the role of myocardial autophagy in experimental non-reflow rats. Methods SD rats were randomly divided into experimental group and control group: coronary artery was ligated for 2 hours and reperfusion for 1 hour. At the end of reperfusion, the reflow area was stained by injecting 6% sulfur 1 m L/kg from the left atrium, and the no reflow area was not stained, and then the coronary artery was religated in situ, and the Evan'S blue was injected into the left atrium in the experimental group, so that the ligation area was blue outside the ligation area and the infarcted area was not blue. The control group was not treated with Evan's blue. Real time quantitative PCR and Western blot were used to detect the expression of autophagy associated protein (LC3,p62/SQSTM1) in myocardium without reflow, infarction and normal myocardium, and ultrastructural changes of autophagy were observed under electron microscope. Results the results of TTC and sulfur staining showed that the no reflow area in the experimental group was significantly higher than that in the control group (P0.05). Expression of autophagy associated protein LC3- 鈪，
本文编号：2213033
本文链接：https://www.wllwen.com/yixuelunwen/jjyx/2213033.html
最近更新
教材专著
