瑞舒伐他汀联合脐血间充质干细胞移植改善急性心肌梗死后心功能

发布时间：2018-09-04 11:42

【摘要】：背景:近年来通过干细胞移植诱导梗死区心肌细胞的再生用以治疗急性心肌梗死后心功能不全,成为较为热议的一种新的治疗手段。目的:观察瑞舒伐他汀联合脐血间充质干细胞移植对大鼠急性心肌梗死后心功能的改善作用。方法:选取45只SD大鼠采用结扎左冠状动脉前降支方法制备心肌梗死模型,随机分为模型组、脐血间充质干细胞移植组和联合组,每组15只。大鼠冠脉结扎后第7天,脐血间充质干细胞移植组及联合组经尾静脉注射脐血间充质干细胞悬液300μL(15.0×108个细胞),模型组注射相同剂量LG-DMEM培养液,联合组给予瑞舒伐他汀1 mg/kg灌胃×28 d(从术后第1天开始灌胃,1次/d),脐血间充质干细胞移植组及模型组同步灌胃相同剂量的生理盐水。造模后5周(细胞移植4周)采用彩色多普勒超声仪检测心功能改善情况,酶联免疫吸附法检测血浆Lp-PLA2水平,苏木精-伊红染色观察心肌组织病理变化;Western blot方法检测心肌梗死区热休克蛋白70表达。结果与结论:1联合组大鼠的左心室射血分数、左心室收缩末压明显高于脐血间充质干细胞移植组(P0.05),脐血间充质干细胞移植组明显高于模型组(P0.05);联合组大鼠的左心室舒张末压明显低于脐血间充质干细胞移植组(P0.05),脐血间充质干细胞移植组明显低于模型组(P0.05);2联合组Lp-PLA2水平明显低于脐血间充质干细胞移植组(P0.05),脐血间充质干细胞移植组明显低于模型组(P0.05);3联合组梗死区的心肌细胞数量较模型组、脐血间充质干细胞移植组明显增加;4心肌梗死区HSP70蛋白表达在联合组明显升高,各组间的表达差异有显著性意义(P0.05);5结果表明,瑞舒伐他汀联合脐血间充质干细胞移植干预后能确切改善心肌梗死大鼠心功能。
[Abstract]:Background: in recent years, the regeneration of myocardial cells in infarcted area induced by stem cell transplantation has become a hot new therapeutic method for the treatment of cardiac insufficiency after acute myocardial infarction. Aim: to observe the effect of resuvastatin combined with umbilical cord blood mesenchymal stem cell transplantation on cardiac function after acute myocardial infarction in rats. Methods: 45 SD rats were randomly divided into three groups: model group, umbilical cord blood mesenchymal stem cell transplantation group and combined group with 15 rats in each group. On the 7th day after coronary artery ligation, the umbilical cord blood mesenchymal stem cell transplantation group and the combined group were injected with 300 渭 L (15.0 脳 108 cells) of mesenchymal stem cell suspension via tail vein. The model group was injected with the same dose of LG-DMEM culture medium. The combined group was given resuvastatin 1 mg/kg intragastrically for 28 days (from the first day after operation), the umbilical cord blood mesenchymal stem cell transplantation group and the model group were given the same dose of normal saline simultaneously. The improvement of cardiac function was detected by color Doppler ultrasound and the plasma Lp-PLA2 level was detected by enzyme-linked immunosorbent assay (Elisa) 5 weeks after the model (4 weeks of cell transplantation). The pathological changes of myocardium were observed by hematoxylin-eosin staining. The expression of heat shock protein 70 in myocardial infarction was detected by Western blot. Results and conclusion the left ventricular ejection fraction (LVEF) of rats in the combined group of 1: 1, The left ventricular end-systolic pressure was significantly higher than that of cord blood mesenchymal stem cell transplantation group (P0.05), the umbilical cord blood mesenchymal stem cell transplantation group was significantly higher than the model group (P0.05), and the left ventricular end-diastolic pressure of the combined group was significantly lower than that of the umbilical cord blood mesenchymal stem cell transplantation group (P0.05). (P0.05), the level of Lp-PLA2 in the umbilical cord blood mesenchymal stem cell transplantation group was significantly lower than that in the model group (P0.05). The level of Lp-PLA2 in the combined group was significantly lower than that in the cord blood mesenchymal stem cell transplantation group (P0.05), and it was significantly lower in the umbilical cord blood mesenchymal stem cell transplantation group than in the model group (P0.05). The number of cells in the model group was higher than that in the model group. In cord blood mesenchymal stem cell transplantation group, the expression of HSP70 protein in myocardial infarction was significantly increased in the combined group, and there was significant difference among the groups (P0.05). Resuvastatin combined with umbilical cord blood mesenchymal stem cell transplantation can improve cardiac function of myocardial infarction rats.
【作者单位】：唐山市开滦总医院心内科;唐山市遵化市人民医院;
【分类号】：R542.22

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