大鼠局灶性脑创伤早期的DTI和~1H-MRS的特征

发布时间：2018-09-04 14:29

【摘要】：目的探讨大鼠局灶性创伤性脑损伤(TBI)早期创伤灶周围区的DTI和1~H-MRS参数随时间的变化。方法健康雄性SD大鼠24只,随机分为创伤组16只、对照组8只。创伤组制作大鼠液压冲击脑皮质损伤动物模型,于两组模型制作后1、3、5h行DTI和1 H-MRS扫描,分别测量伤侧病灶周围区及健侧半球对应部位的DTI参数ADC值、FA值和1 H-MRS参数N-乙酰天门冬氨(NAA)/肌酸(Cr)、胆碱类化合物(Cho)/Cr和乳酸(Lac)/Cr,对照组测量双侧大脑半球相应部位各指标,并进行统计学分析。结果创伤组伤后1、3、5h创伤侧病灶周围区与健侧对应部位比较,ADC值均降低、FA值均升高(P均0.05);NAA/Cr均降低、Lac/Cr均升高(P均0.05),Cho/Cr差异无统计学意义(P均0.05)。创伤灶周围区伤后3h较1h的ADC值降低(P0.001)、FA值升高(P=0.002),伤后5h较3h的ADC值升高(P=0.005)、FA值降低(P=0.012)。对照组双侧大脑半球各时间点各指标差异均无统计学意义(P均0.05)。结论大鼠脑皮质局灶性损伤后1~5h创伤灶周围区存在微观结构及代谢变化,创伤灶周围区的继发性脑损伤于伤后1h已发生,而伤后3h可能有所加重。
[Abstract]:Objective to investigate the changes of DTI and 1~H-MRS parameters in early traumatic brain injury (TBI) in rats. Methods 24 healthy male SD rats were randomly divided into trauma group (n = 16) and control group (n = 8). The rat model of cerebral cortex injury induced by hydraulic percussion was made in the trauma group. DTI and 1 H-MRS scans were performed at 1: 3 and 5 hours after the establishment of the two groups. The DTI parameters (ADC value, FA value and 1 H-MRS parameter, (Cho) / Cr and (Lac) / Cr) were measured in the peripheral region of the injured lesion and the corresponding parts of the contralateral hemispheres, respectively. The indexes of the corresponding parts of the cerebral hemispheres in the control group were measured, and the corresponding parameters of (Cho) / Cr and (Lac) / Cr lactate were measured in both cerebral hemispheres of the control group. Statistical analysis was carried out. Results in the trauma group, the ADC values in the perifocal area of the trauma side were significantly lower than those in the normal side at 5 h after injury (P 0.05). The values of NAA / Cr in the trauma group were significantly lower than those in the contralateral side (P 0.05). There was no significant difference in the value of Cho-P / Cr between the two groups (P 0.05). The ADC value in the peri-traumatic area decreased (P0. 001) and the FA value increased (P0. 002) at 3 h after injury, and the ADC value increased (P0. 005) at 5 h after injury (P0. 005) and decreased (P0. 012). In the control group, there was no significant difference in the indexes at all time points in the bilateral cerebral hemispheres (P 0.05). Conclusion the microstructures and metabolism changes in the peri-traumatic area of rat cerebral cortex were observed at 1h and 5h after focal injury. The secondary brain injury occurred at 1h after injury, and may be aggravated at 3h after injury.
【作者单位】：首都医科大学附属北京天坛医院超声科;北京市神经外科研究所;首都医科大学附属北京天坛医院放射科;
【分类号】：R651.15;R445.2

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