骨髓间质干细胞对大鼠脑损伤后血管再生的影响

发布时间：2018-11-10 23:11

【摘要】：目的:探讨骨髓间质干细胞(bone marrow mesenchymal stem cells,BM-MSCs)对大鼠颅脑损伤后血管再生的影响。方法:自由落体法建立大鼠脑撞击伤模型,50只Sprague-Dawley大鼠随机分为移植组和对照组,每组25只。移植组大鼠手术后12 h侧脑室注射法移植BM-MSCs,对照组仅注射生理盐水。术后第1,3,7,14和21天行改良大鼠神经功能缺损评分(modified neurological deficit scores of rats,mNSS)。于术前,术后3,6,12,24 h和3,7 d采用流式细胞仪检测大鼠外周血CD34和CD133抗体双标记细胞。免疫组织化学SP法检测损伤周围脑组织CD31和神经元特异性烯醇化酶(neuron-specific enolase,NSE)的表达。结果:两组间mNSS分值差异有统计学意义(F=5.997,P0.05),组内不同时间点间差异也有统计学意义(F=37.106,P0.01)。术后第7,14,21天对照组较移植组mNSS评分高,差异有统计学意义(均P0.05)。大鼠外周血中存在CD34和CD133双阳性细胞表达。对照组大鼠外周血中CD34和CD133双阳性细胞数伤后3 h为下降状态,后升高,伤后6 h达最高点,此后逐渐下降,伤后24 h降至正常水平。移植组有同样的趋势,CD34和CD133双阳性细胞升高持续到伤后24 h,其数量明显高于对照组(P0.05)。术前两组NSE均有阳性表达,术后7,14 d时移植组的NSE表达显著高于对照组(P0.05)。术前两组CD31的阳性表达很少,术后3,7 d时移植组的CD31表达显著高于对照组(均P0.05)。结论:BM-MSCs移植可以增加脑创伤后大鼠外周血中内皮祖细胞数量并持续约24 h,可以调高大鼠脑创伤后损伤周围区的血管生成标志物的表达和神经元标志物的表达。BM-MSCs移植组大鼠脑损伤后神经功能较对照组改善明显。
[Abstract]:Objective: to investigate the effect of bone marrow mesenchymal stem cells (bone marrow mesenchymal stem cells,BM-MSCs) on vascular regeneration after craniocerebral injury in rats. Methods: the rat model of brain impact injury was established by free-fall method. Fifty Sprague-Dawley rats were randomly divided into transplantation group and control group with 25 rats in each group. Rats in the transplantation group received intraventricular injection of saline 12 hours after operation. The modified neurological deficit score (modified neurological deficit scores of rats,mNSS) was performed on the 1st day, 3rd day, 14 th and 21 th day after operation. CD34 and CD133 antibody double labeled cells in peripheral blood of rats were detected by flow cytometry at 24 hours and 3 days after operation. The expression of CD31 and neuron-specific enolase (neuron-specific enolase,NSE) were detected by immunohistochemical SP method. Results: there was significant difference in mNSS score between the two groups (P 0.05), and there was also significant difference between the two groups at different time points (P 0.01). The mNSS score of the control group was significantly higher than that of the transplantation group on day 7, 14 and 21 (P0.05). CD34 and CD133 double positive cells were expressed in peripheral blood of rats. In the control group, the number of CD34 and CD133 double positive cells in peripheral blood decreased 3 h after injury, then increased, reached the peak at 6 h after injury, then gradually decreased, and then decreased to normal level 24 h after injury. There was the same trend in the transplantation group, CD34 and CD133 double positive cells increased until 24 hours after injury, the number of the cells was significantly higher than that of the control group (P0.05). There was positive expression of NSE in both groups before operation, and the expression of NSE in transplantation group was significantly higher than that in control group at 714 days postoperatively (P0.05). The expression of CD31 in the transplantation group was significantly higher than that in the control group 3 days after operation (P0.05). Conclusion: BM-MSCs transplantation can increase the number of endothelial progenitor cells in peripheral blood of rats after brain trauma for 24 hours. The expression of angiogenic markers and neuronal markers in the peritraumatic area of high and high brain injury could be regulated. The nerve function in the BM-MSCs transplantation group was significantly improved than that in the control group.
【作者单位】：九江市第一人民医院神经外科;
【基金】：江西省卫生厅科技基金(20131736)~~
【分类号】：R651.15

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