羊脱细胞真皮基质异种移植后白介素4和γ-干扰素的表达

发布时间：2018-11-15 21:45

【摘要】：目的:评价自制的羊脱细胞真皮基质(acellular dermal matrix,ADM)的免疫原性。方法:自制羊ADM,然后制成交联型ADM和未交联型ADM。将160只大鼠随机分配为4组:A组,于大鼠皮下埋植未交联型羊ADM;B组,于大鼠皮下埋植交联型羊ADM;C组,于大鼠皮下埋植异体皮;对照组,大鼠做皮下游离切开后无埋植物直接缝合。观察实验组埋植区外观变化,有无明显的排斥反应,取埋植区组织显微镜下观察局部的炎症反应情况。埋植后3 d及1,2,3周检测各组大鼠血清中白介素4(IL-4)和γ-干扰素(IFN-γ)的表达情况。结果:肉眼观察埋植区切口愈合良好,无明显的免疫排斥反应,羊ADM逐渐被吸收减少。镜下观察羊ADM与周围的组织连接较紧密,有一定量的炎性细胞浸润至羊ADM深处并有增多趋势,羊ADM结构逐渐被破坏。A,B,C组和对照组的IL-4和IFN-γ的表达在第3天差异无统计学意义(P0.05)。3 d后对照组创面炎症反应消退,IL-4和IFN-γ的表达下降明显,而A,B,C组的表达仍然处于高位,体现出羊ADM的埋植使IL-4和IFN-γ的表达增高,与羊ADM引起的免疫反应有关。IL-4/IFN-γ的值显示:与对照组相比,3 d内A,B,C组差异无统计学意义(P0.05),1周、2周、3周差异均有统计学意义(P0.05),A,B,C组之间差异无统计学意义(P0.05)。结论:自制的羊ADM移植后引起的排斥反应强度较低,具有较低的免疫原性;交联和未交联羊ADM对IL-4和IFN-γ的表达的影响无明显差异。
[Abstract]:Objective: to evaluate the immunogenicity of goat acellular dermal matrix (acellular dermal matrix,ADM). Methods: sheep ADM, was made into crosslinked ADM and uncrosslinked ADM.. One hundred and sixty rats were randomly divided into four groups: group A, uncross-linked sheep ADM;B group, ADM;C group and allogeneic skin implantation; In the control group, no buried plants were sutured directly after subcutaneous free incision. The appearance and rejection of the implanted area in the experimental group were observed, and the local inflammatory reaction was observed under the tissue microscope of the implanted area. The expression of interleukin-4 (IL-4) and interferon 纬 (IFN- 纬) in serum of rats in each group were detected 3 days after implantation and 3 weeks after implantation. Results: the incision healed well and there was no obvious immune rejection. ADM was gradually absorbed and decreased. Under microscope, sheep ADM was closely connected with surrounding tissues, and a certain number of inflammatory cells infiltrated deep into sheep ADM and tended to increase. The structure of sheep ADM was destroyed gradually. There was no significant difference in the expression of IL-4 and IFN- 纬 between the C group and the control group on the 3rd day (P0.05). After 3 days, the inflammatory reaction of the wound surface in the control group disappeared, the expression of IL-4 and IFN- 纬 decreased significantly, while the expression of IFN- 纬 decreased significantly. The expression of IL-4 and IFN- 纬 in group C was still high, which indicated that the expression of IL-4 and IFN- 纬 was increased by implantation of sheep ADM, which was related to the immunological response induced by ADM. The value of IL-4/IFN- 纬 showed that compared with the control group, the expression of IL-4 and IFN- 纬 increased within 3 days. There was no significant difference in group C (P0.05), 1 week, 2 weeks, 3 weeks difference was statistically significant (P0.05), there was no significant difference between group C (P0.05). Conclusion: the intensity of rejection induced by self-made sheep ADM transplantation is lower and the immunogenicity is lower, and there is no significant difference between cross-linked and uncross-linked sheep ADM on the expression of IL-4 and IFN- 纬.
【作者单位】：中南大学湘雅医院烧伤科;
【基金】：内蒙古自然科学基金(2008040MS1115)~~
【分类号】：R644

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