大鼠全脑缺血再灌注后不同时间点给予纯氧对脑的影响的研究

发布时间：2018-11-18 10:59

【摘要】：目的：通过制作大鼠全脑缺血模型，比较再灌注后不同时间给予纯氧对脑损伤的影响，从而探索脑缺血再灌注后给予高浓度氧的安全时间范围。方法：健康SD雄性大鼠48只随机分成6组：假手术组（Sham，n=8，吸入空气），纯氧组（n=8，再灌注后立即给予纯氧1h），纯氧一组（n=8，再灌注0.5h后给予纯氧1h），纯氧二组（n=8，再灌注1h后给予纯氧1h），纯氧三组（n=8，再灌注2h后给予纯氧1h），空气组（n=8，，一直吸入空气）。参照Pulsinelli四血管阻断法制作大鼠全脑缺血再灌注模型，全脑缺血15分钟。在缺血前（0H），再灌注后(0.5H，1H，2H，4H)采右侧颈内静脉血，离心取血清，测定异构前列腺素（8-iso-PGF2α）、S-100B浓度；再灌注24小时后断头取脑，沿中线将大脑分半，一半测定脑水含量，另一半4%多聚甲醛固定，制成石蜡切片，通过TUNEL染色观察海马CA1区神经元损伤情况。结果：纯氧组和纯氧一组在给予纯氧后，颈内静脉8-iso-PGF2α、S-100B蛋白水平及神经元凋亡百分比、脑水含量均比空气组高，差异具有统计学意义；纯氧二组在给予纯氧后，除S-100B水平，其他指标与空气组有统计学差异，且除异构前列腺素，其他指标与纯氧三组无统计学差异；纯氧三组上述指标与空气组均无统计学差异。结论：在全脑缺血再灌注早期（1小时内）给予纯氧会通过加强氧化应激反应而加重脑损伤，而在再灌注2小时后再给予可能影响较小。
[Abstract]:Aim: to study the effect of pure oxygen on brain injury in rats with global cerebral ischemia and to explore the safe time range of high concentration oxygen after cerebral ischemia reperfusion. Methods: Forty-eight healthy SD male rats were randomly divided into six groups: sham operation group (Sham,n=8, inhaled air), pure oxygen group (n = 8), pure oxygen group (n = 1 h after 0.5 h reperfusion), pure oxygen group (n = 8), pure oxygen group (n = 8). Pure oxygen group 2 (nil 8, 1 h after reperfusion), pure oxygen group 3 (na 8, 2 h after reperfusion for 1 h), air group (n 8, inhaled air all the time). The rat model of global cerebral ischemia reperfusion was established by Pulsinelli four-vessel occlusion method, and the whole brain was ischemia for 15 minutes. Before ischemia (0H) and after reperfusion (0.5H), the right jugular vein blood was collected and the serum was centrifuged to determine the concentration of isoprostaglandin (8-iso-PGF2 伪) and S-100B; After 24 hours of reperfusion, the head was cut off, the brain was divided into half along the midline, the water content in the brain was measured in half, and the other half was fixed with 4% paraformaldehyde. The brain was made into paraffin sections. The neuronal damage in the CA1 area of hippocampus was observed by TUNEL staining. Results: the levels of 8-iso-PGF2 伪, S-100B protein and the percentage of neuronal apoptosis in the jugular vein of the pure oxygen group and the pure oxygen group were higher than those in the air group, and the difference was statistically significant. After pure oxygen was given, the other indexes were significantly different from those of the air group except S-100B, and there was no significant difference between the other indexes and the pure oxygen group except isomeric prostaglandins. There was no statistical difference between the above indexes of pure oxygen group and air group. Conclusion: at the early stage (within 1 hour) of global cerebral ischemia-reperfusion, pure oxygen may aggravate the brain injury by strengthening the oxidative stress response, but it may have little effect after 2 hours of reperfusion.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R651.15

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