运动训练对中度脑损伤大鼠学习记忆能力的影响及机制研究
发布时间：2018-11-18 11:00
【摘要】：目的:观察运动训练对TBI后大鼠空间学习记忆能力的影响,对TBI大鼠模型进行规律足量运动训练后,观察各组大鼠的水迷宫表现来探讨运动训练对学习记忆能力的影响。通过研究大鼠海马区BDNF的表达情况,探讨运动训练影响TBI大鼠学习记忆能力的作用机制。方法:成年雄性SD大鼠45只,随机分为对照组(假手术+非运动训练)、创伤组(手术+非运动训练)和创伤训练组(手术+运动训练),每组15只。创伤组和创伤训练组建立TBI模型,TBI后第4天,对创伤训练组进行运动训练(网屏训练、平衡木训练和游泳训练),每一项目每天训练2次,每次10min,持续2周。运动训练结束后,对各组大鼠行Morris水迷宫实验,以评估大鼠的空间学习记忆能力的变化,记录第1-4天大鼠逃避潜伏期时间和第5天大鼠穿越平台次数。水迷宫实验结束以后,处死大鼠,获取大脑海马组织,采用免疫组织化学方法对大鼠海马组织CA1区脑源性神经营养因子(BDNF)的阳性细胞进行染色,并测其阳性细胞表达数来表示BDNF的表达情况。采用SPSS20.0版统计软件分析实验数据,本研究计量资料以(`x±s)表示,组间比较采用单因素方差分析,P0.05为差异有统计学意义。结果:水迷宫实验结果:定位航行实验:与创伤组相比,对照组和创伤训练组大鼠第2天至第4天逃避潜伏期时间明显缩短(P0.05),对照组和创伤训练组无明显差异(P0.05)。空间探索实验:对照组和创伤训练组大鼠穿越平台次数明显高于创伤组(P0.05),对照组和创伤训练组则无明显差异(P0.05)。免疫组化结果显示:对照组和创伤组BDNF表达无明显差异(P0.05),创伤训练组BDNF表达明显高于创伤组和对照组(P0.05)。结论:TBI后大鼠可出现学习的记忆能力的下降,而足量规律的运动训练则以可改善TBI所致学习记忆能力障碍,其作用机制可能与运动训练可以使大鼠海马区BDNF表达增高有关。
[Abstract]:Aim: to observe the effect of exercise training on the spatial learning and memory ability of rats after TBI, and to observe the water maze performance of rats in each group after regular and adequate exercise training in TBI rats to explore the effect of exercise training on learning and memory ability. By studying the expression of BDNF in the hippocampus of rats, the mechanism of the effect of exercise training on the learning and memory ability of TBI rats was discussed. Methods: Forty-five adult male SD rats were randomly divided into three groups: the control group (sham operation non-exercise training), the trauma group (operation non-exercise training) and the trauma training group (operation training), with 15 rats in each group. The TBI model was established in the trauma group and the trauma training group. On the 4th day after TBI, the trauma training group was given exercise training (screen training, balance beam training and swimming training). Each item was trained twice a day for 10 minutes for 2 weeks. After exercise training, Morris water maze test was performed to evaluate the spatial learning and memory ability of rats, and to record the escape latency time of rats on day 1-4 and the times of crossing the platform on day 5. After the water maze experiment, the rats were killed and the hippocampal tissues were obtained. The positive cells of brain-derived neurotrophic factor (BDNF) in the CA1 region of the hippocampus of the rats were stained by immunohistochemical method. The number of positive cells was measured to indicate the expression of BDNF. The statistical software of SPSS20.0 was used to analyze the experimental data. The measurement data of this study were expressed as (x 卤s), and the analysis of variance was used in the comparison between groups. The difference was statistically significant (P0.05). Results: the result of water maze experiment: compared with the trauma group, the escape latency of rats in the control group and the trauma training group was significantly shorter than that in the trauma group (P0.05). There was no significant difference between the control group and the trauma training group (P0.05). Space exploration experiment: the times of traversing the platform in the control group and trauma training group were significantly higher than those in the trauma group (P0.05), but there was no significant difference between the control group and the trauma training group (P0.05). Immunohistochemical results showed that there was no significant difference in BDNF expression between the control group and the trauma group (P0.05). The expression of BDNF in the trauma training group was significantly higher than that in the trauma group and the control group (P0.05). Conclusion: there is a decrease in learning memory ability after TBI in rats, while adequate exercise training can improve the learning and memory impairment induced by TBI. The mechanism may be related to the increase of BDNF expression in hippocampus of rats after exercise training.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R651.15
本文编号：2339847
[Abstract]:Aim: to observe the effect of exercise training on the spatial learning and memory ability of rats after TBI, and to observe the water maze performance of rats in each group after regular and adequate exercise training in TBI rats to explore the effect of exercise training on learning and memory ability. By studying the expression of BDNF in the hippocampus of rats, the mechanism of the effect of exercise training on the learning and memory ability of TBI rats was discussed. Methods: Forty-five adult male SD rats were randomly divided into three groups: the control group (sham operation non-exercise training), the trauma group (operation non-exercise training) and the trauma training group (operation training), with 15 rats in each group. The TBI model was established in the trauma group and the trauma training group. On the 4th day after TBI, the trauma training group was given exercise training (screen training, balance beam training and swimming training). Each item was trained twice a day for 10 minutes for 2 weeks. After exercise training, Morris water maze test was performed to evaluate the spatial learning and memory ability of rats, and to record the escape latency time of rats on day 1-4 and the times of crossing the platform on day 5. After the water maze experiment, the rats were killed and the hippocampal tissues were obtained. The positive cells of brain-derived neurotrophic factor (BDNF) in the CA1 region of the hippocampus of the rats were stained by immunohistochemical method. The number of positive cells was measured to indicate the expression of BDNF. The statistical software of SPSS20.0 was used to analyze the experimental data. The measurement data of this study were expressed as (x 卤s), and the analysis of variance was used in the comparison between groups. The difference was statistically significant (P0.05). Results: the result of water maze experiment: compared with the trauma group, the escape latency of rats in the control group and the trauma training group was significantly shorter than that in the trauma group (P0.05). There was no significant difference between the control group and the trauma training group (P0.05). Space exploration experiment: the times of traversing the platform in the control group and trauma training group were significantly higher than those in the trauma group (P0.05), but there was no significant difference between the control group and the trauma training group (P0.05). Immunohistochemical results showed that there was no significant difference in BDNF expression between the control group and the trauma group (P0.05). The expression of BDNF in the trauma training group was significantly higher than that in the trauma group and the control group (P0.05). Conclusion: there is a decrease in learning memory ability after TBI in rats, while adequate exercise training can improve the learning and memory impairment induced by TBI. The mechanism may be related to the increase of BDNF expression in hippocampus of rats after exercise training.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R651.15
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