不同液体复苏失血性休克大鼠对其骨髓中髓系抑制细胞数量变化的影响

发布时间：2018-11-24 13:31

【摘要】：目的：探究用不同液体复苏失血性休克大鼠后，不同时间点其骨髓中髓系抑制性细胞(myeloid-derivedsuppressorcells,MDSCs)的数量变化情况。方法：采用股动脉放血方法建立失血性休克大鼠模型，在液体复苏早期阶段懫用乳酸林格氏液、晶胶液、7.5%高渗氯化钠三种不同液体复苏，其后为自体血液回输阶段，在复苏成功后12h、24h和48h三个时间点用脊髓离断法处死大鼠，同时设假手术组正常对照组。处死后，获取大鼠骨髓细胞悬液，采用流式细胞技术观察大鼠骨髓中髓系抑制性细胞的数量随时间变化的情况。结果： 1.假手术组骨髓中MDSCs含量随时间的延长无明显差异，采用单因素方差分析，，无统计学意义（P0.05）；乳酸林格氏液组、晶胶液组和高渗氯化钠组各组骨髓中MDSCs含量24小时时间点较12小时时间点升高，48小时时间点较24小时时间点降低，12小时与48小时骨髓中MDSCs含量相近似，无统计学差异（P0.05）。 2.与假手术组相比，各时间点的乳酸林格氏液组、晶胶液组及高渗氯化钠组骨髓中MDSCs所占的比例均发生明显变化，且具有统计学差异（P0.05）； 3.乳酸林格氏液组、晶胶液组及高渗氯化钠组三组间比较，高渗氯化钠组较乳酸林格氏液组和晶胶液组均明显降低，有统计学意义（P0.05），乳酸林格氏液组和晶胶液组之间骨髓中MDSCs含量较为接近，无明显差异，无统计学意义（P0.05）。结论： 1．失血性休克早期液体复苏后，大鼠骨髓中MDSCs的数量随时间的延长先升高后降低，说明液体复苏可能适度抑制MDSCs的数量。 2．与乳酸林格氏液、晶胶液相比，高渗盐溶液复苏失血性休克时，能够更有效抑制骨髓中MDSCs的数量，对失血性休克后的免疫调节作用较强。
[Abstract]:Aim: to investigate the changes of the number of myeloid inhibitory cells (myeloid-derivedsuppressorcells,MDSCs) in bone marrow of hemorrhagic shock rats resuscitated with different fluids at different time points. Methods: the rat model of hemorrhagic shock was established by femoral artery bleeding. In the early stage of fluid resuscitation, the rats were resuscitated with three different fluids: Ringer's lactate solution, crystal glue solution and 7.5% hypertonic sodium chloride, followed by autologous blood transfusion. The rats were killed by spinal cord amputation at 24 h and 48 h after successful resuscitation, and the sham operation group was set up as normal control group. After the rats were killed, bone marrow cell suspension was obtained. Flow cytometry was used to observe the changes of the number of medullary inhibitory cells in rat bone marrow with time. Results: 1. There was no significant difference in bone marrow MDSCs content with the prolongation of time in sham-operation group, but there was no statistical significance by univariate ANOVA (P0.05). The MDSCs content in bone marrow of Ringer's lactate group, crystal gel solution group and hypertonic sodium chloride group was higher than that of 12 hour time point, but decreased at 48 hours time point, and was similar to that of 48 hour bone marrow. There was no statistical difference (P0.05). 2. Compared with sham operation group, the percentage of MDSCs in bone marrow of Ringer's lactate group, crystal gel solution group and hypertonic sodium chloride group were significantly different at each time point (P0.05); Compared with Ringer's lactate solution group, crystal gel solution group and hypertonic sodium chloride group, hypertonic sodium chloride group was significantly lower than that of lactate Ringer's liquid group and crystal gel solution group (P0.05). The content of MDSCs in bone marrow of Ringer's lactate group and crystal gel group was similar, no significant difference (P0.05). Conclusion: 1. After early fluid resuscitation in hemorrhagic shock, the number of MDSCs in bone marrow of rats increased first and then decreased with the prolongation of time, indicating that fluid resuscitation may moderately inhibit the amount of MDSCs. 2. 2.Compared with Ringer's lactate solution and crystal gel solution, hyperosmotic salt solution was more effective in inhibiting the amount of MDSCs in bone marrow during hemorrhagic shock resuscitation, and had stronger immunomodulation effect after hemorrhagic shock.
【学位授予单位】：蚌埠医学院
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R459.7

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