小檗碱对颅脑创伤模型小鼠对侧顶叶皮层继发性损伤的影响

发布时间：2018-12-16 06:13

【摘要】：目的观察小鼠颅脑创伤(traumatic brain injury,TBI)后对侧皮层的炎症反应、氧化损伤及神经元丢失的情况,并探讨盐酸小檗碱对这种继发性损害的影响。方法采用自由落体撞击法建立TBI小鼠模型,通过灌胃给予盐酸小檗碱[50mg/(kg·d)],共21 d。采用免疫荧光染色观察对侧顶叶皮层小胶质细胞、星形胶质细胞的增殖活化情况,免疫组化染色观察对侧皮层COX-2(cyclooxygenase-2)、i NOS(inducible nitric oxide synthase)的表达情况以及DNA氧化损伤和神经元丢失的情况。结果 TBI导致对侧顶叶皮层小胶质细胞和星形胶质细胞的增殖活化、COX-2和i NOS的表达以及DNA氧化损伤较对照组明显增加,分别为(19.82±1.88)和(16.96±1.69)、(13.79±4.32)和(8.67±0.96)、(27.86±5.38)和(16.00±7.59)、(31.92±6.57)和(24.79±2.78)(P0.01或P0.05),但神经元数量无显著丢失,分别为(49.05±4.38)和(48.56±3.56)(P0.05);小檗碱显著抑制对侧皮层小胶质细胞、COX-2和i NOS的表达,分别为(15.49±1.88)和(19.82±1.88)、(16.83±7.89)和(27.86±5.38)、(26.25±2.41)和(31.92±6.57)(P0.01或P0.05),而星形胶质细胞和DNA氧化损伤的比较差异无统计学意义,分别为(11.91±1.42)和(13.79±4.32)、(32.82±2.78)和(34.40±2.41)(P0.05)。结论 TBI导致远隔对侧顶叶皮层继发性损害较轻,仅导致炎症反应、氧化损伤增加,但并不会导致远隔对侧皮层神经元丢失;盐酸小檗碱能够显著抑制TBI后对侧皮层的炎症反应。
[Abstract]:Objective to observe the inflammatory response, oxidative injury and neuron loss in the contralateral cortex after (traumatic brain injury,TBI in mice, and to investigate the effect of berberine hydrochloride on the secondary injury. Methods the TBI mouse model was established by free falling body impingement. Berberine hydrochloride [50mg/ (kg d)] was administered intragastrically for 21 days. The proliferation and activation of microglia and astrocytes in contralateral parietal cortex were observed by immunofluorescence staining. COX-2 (cyclooxygenase-2) in contralateral cortex was observed by immunohistochemical staining. The expression of i NOS (inducible nitric oxide synthase) and DNA oxidative damage and neuronal loss. Results TBI induced the proliferation and activation of microglia and astrocytes in the contralateral parietal cortex. The expression of COX-2 and I NOS and the oxidative damage of DNA were significantly increased (19.82 卤1.88) and (16.96 卤1.69), respectively. (13.79 卤4.32) and (8.67 卤0.96), (27.86 卤5.38), (16.00 卤7.59), (31.92 卤6.57) and (24.79 卤2.78) (P0.01 or P0.05), but the number of neurons was not significantly lost. (49.05 卤4.38) and (48.56 卤3.56) (P0.05); Berberine significantly inhibited the expression of COX-2 and I NOS in contralateral cortical microglia (15.49 卤1.88) and (19.82 卤1.88), (16.83 卤7.89) and (27.86 卤5.38), respectively. (26.25 卤2.41) and (31.92 卤6.57) (P0.01 or P0.05), while there was no significant difference in oxidative damage between astrocytes and DNA (11.91 卤1.42) and (13.79 卤4.32), respectively. (32.82 卤2.78) and (34.40 卤2.41) (P0.05). Conclusion the secondary injury of parietal cortex in contralateral parietal cortex induced by TBI was mild, but the oxidative damage was increased, but not the loss of neurons in contralateral cortex, and berberine hydrochloride could significantly inhibit the inflammatory response of contralateral cortex after TBI.
【作者单位】：汕头大学医学院附属粤北人民医院神经内科;深圳罗湖区人民医院神经内科;中山大学附属第一医院神经科;
【基金】：广东省自然科学基金面上项目(编号:S2013010015786) 韶关市科技计划项目[编号:韶科(卫)2010-01]
【分类号】：R651.15;R-332

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