miR-126对急性心肌梗死大鼠心肌细胞凋亡的影响

发布时间：2018-12-17 21:09

【摘要】：目的探讨miR-126对急性心肌梗死(AMI)大鼠心肌细胞凋亡的影响。方法通过冠状动脉左前降支结扎大鼠建立AMI模型,随机分为AMI组、miR-126 mimics negative control(NC)组及miR-126组,其中NC组及miR-126组分别采用心肌组织局部注射慢病毒转染miR-126 mimics NC及miR-126 mimics,另外假手术组采用冠状动脉左前降支穿线不结扎。记录大鼠心脏功能,TCC法及原位末端凋亡法(TUNEL)检测心肌凋亡指数及梗死面积,比色法检测天冬氨酸蛋白水解酶(Caspase 3)及Caspase 8活性,Western-blot法检测Bax及Bcl-2表达,同时RTPCR法检测Fas及Fas-L mRNA表达。结果与假手术组比较,AMI组及NC组左室射血分数(LVEF)及左室长轴缩短分数(FS)升高,左室舒张末期内径(LVDd)及左室收缩末期内径(LVDs)降低,心肌凋亡指数提高,心肌梗死面积增大,Caspase 3及Caspase 8活性上升,Bax蛋白表达量上调,Bcl-2蛋白表达量下调,Fas及Fas-L mRNA水平上升,差异具有统计学意义(P0.01)。与AMI组及NC组比较,miR-126组能扭转此变化,差异具有统计学意义(P0.01)。结论 miR-126能显著抑制AMI大鼠心肌细胞凋亡,与调节细胞凋亡相关蛋白表达有关。
[Abstract]:Objective to investigate the effect of miR-126 on cardiomyocyte apoptosis in rats with acute myocardial infarction (AMI). Methods AMI model was established by left anterior descending coronary artery ligation in rats. The rats were randomly divided into AMI group, miR-126 mimics negative control (NC) group and miR-126 group. The NC group and the miR-126 group were treated with miR-126 mimics NC and miR-126 mimics, respectively by local injection of lentivirus into the myocardium. In the sham operation group, the left anterior descending coronary artery was not ligated. Cardiac function was recorded, myocardial apoptosis index and infarct size were measured by TCC and in situ terminal apoptosis (TUNEL), aspartate protein hydrolase (Caspase 3) and Caspase 8 activity were detected by colorimetry, Bax and Bcl-2 expression were detected by Western-blot assay. The expression of Fas and Fas-L mRNA was detected by RTPCR. Results compared with sham operation group, left ventricular ejection fraction (LVEF) and left ventricular long axis shortening fraction (FS) were increased, left ventricular end-diastolic diameter (LVDd) and left ventricular end-systolic diameter (LVDs) were decreased and myocardial apoptosis index was increased in AMI and NC groups. The area of myocardial infarction increased, the activity of Caspase 3 and Caspase 8 increased, the expression of Bax protein increased, the expression of Bcl-2 protein decreased, and the level of Fas and Fas-L mRNA increased. The difference was statistically significant (P0.01). Compared with AMI group and NC group, miR-126 group could reverse the change, the difference was statistically significant (P0.01). Conclusion miR-126 can significantly inhibit cardiomyocyte apoptosis in AMI rats, which is related to the regulation of apoptosis-related protein expression.
【作者单位】：华中科技大学同济医学院附属普爱医院心胸外科;
【分类号】：R542.22

【相似文献】