人膝关节滑膜细胞的增殖、分泌和趋化的体外研究

发布时间：2019-04-01 18:55

【摘要】：第一部分人膝关节滑膜细胞的体外培养 目的：获得人滑膜B型细胞,进行后续研究。方法：提取滑膜组织,消化,培养,传代,鉴定。结果：获得人滑膜B型细胞。结论：本方法可以获得人滑膜B型细胞,满足实验的需求。 第二部分tTG. β-NGF及bFGF对人膝关节滑膜细胞的作用 目的：研究tTG、βNGF及bFGF对人膝关节滑膜B型细胞的作用。方法：将第一部分培养好的滑膜细胞铺板,加入不同浓度的tTG、β-NGF及bFGF,抽取上清,测Ⅰ型胶原蛋白的含量,加入MTT,测细胞增殖,利用Transwel l测趋化作用。结果：tTG在20ng/ml之前,对滑膜B型细胞有促进增殖、分泌作用,之后,随着浓度的增加,抑制细胞增殖和分泌Ⅰ型胶原蛋白,tTG对滑膜B型细胞无趋化作用。β-NGF对滑膜B型细胞有抑制增殖和分泌Ⅰ型胶原蛋白作用,β-NGF对滑膜B型细胞无趋化作用。bFGF对滑膜B型细胞有促进增殖、分泌作用,bFGF对滑膜B型细胞无趋化作用。结论：在滑膜B型细胞增殖和分泌Ⅰ型胶原蛋白方面,tTG有双重作用,β-NGF有抑制作用,bFGF有促进作用；在滑膜B型细胞趋化方面,三种均无影响。
[Abstract]:Objective: to obtain human synovial type B cells for follow-up study. Methods: synovial tissue was extracted, digested, cultured, subcultured and identified. Results: human synovial type B cells were obtained. Conclusion: the human synovial type B cells can be obtained by this method, which can meet the needs of the experiment. Part two tTG. Effects of 尾-NGF and bFGF on human synovial cells objective: to study the effects of tTG, 尾 NGF and bFGF on human synovial type B cells. Methods: the synovial cells were cultured in the first part. Different concentrations of tTG, 尾-NGF and bFGF, were added to extract supernatant. The content of type 鈪，

本文编号：2451794